| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,582,398 | T→C | 16.6% | S173S (AGT→AGC) | gadX → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,582,398 | 0 | T | C | 16.6% | 202.9 / 25.5 | 125 | S173S (AGT→AGC) | gadX | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (53/51); new base C (8/13); total (61/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 126 total aligned reads displayed.
AATATCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCA > minE/2582339‑2582466 | aataTCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTaaa > 1:3162899/1‑68 (MQ=255)aataTCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTaaa > 1:2467974/1‑68 (MQ=255)aataTCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTaaa > 1:224629/1‑68 (MQ=255) atatCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTaaa > 1:3025554/1‑67 (MQ=255) atatCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAg > 1:2099667/1‑68 (MQ=255) atatCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAg > 1:2109040/1‑68 (MQ=255) atatCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAg > 1:2277068/1‑68 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:1586132/1‑69 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:3203505/1‑69 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:1874561/1‑69 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:2200078/1‑69 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:422994/1‑69 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:1332692/1‑69 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:826716/1‑69 (MQ=255) tCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaa > 1:761022/1‑68 (MQ=255) aGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGaaaa > 1:1127630/1‑60 (MQ=255) tGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCga > 1:2591137/1‑68 (MQ=255) tGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCga > 1:1251081/1‑68 (MQ=255) tGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCga > 1:737338/1‑68 (MQ=255) ggACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCgaag < 1:1997238/69‑1 (MQ=255) ggACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCgaag < 1:1329664/69‑1 (MQ=255) ggACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCgaag < 1:417702/69‑1 (MQ=255) ggACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCgaag < 1:2245601/69‑1 (MQ=255) aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAGAAATTGCGCGAAGAAGAGAc < 1:2374208/69‑1 (MQ=255) aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAGAAATTGCGCGAAGAAGAGAc < 1:167910/69‑1 (MQ=255) aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAGAAATTGCGCGAAGAAGAGAc < 1:2959743/69‑1 (MQ=255) aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAgaga > 1:2441034/1‑68 (MQ=255) aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAc > 1:178768/1‑69 (MQ=255) aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAc > 1:3072214/1‑69 (MQ=255) gCCCGAATCGCCAGCGAGCTGTTGATTAGTCCAAGTCTGTTAAAGAAGAAATTGCGCGAAGAAGAGAc < 1:1795680/68‑1 (MQ=255) cGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca < 1:2700881/69‑1 (MQ=255) gAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatcat < 1:9394/69‑1 (MQ=255) gAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatcat < 1:1389000/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:1065568/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:409189/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:2949123/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:3150758/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:1449535/69‑1 (MQ=255) tCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:2089172/1‑69 (MQ=255) tCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:1853104/1‑69 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGGAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:2747822/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:3219800/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:908036/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:780311/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:467565/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:1727380/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:3227288/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:839444/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:3415282/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:28303/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2817483/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2659464/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1314138/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1814612/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2611066/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:188726/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2087421/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1163261/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTCTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1959453/67‑1 (MQ=255) gCCAGCGAGCTATTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:765152/1‑67 (MQ=255) gCCACCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:300261/1‑63 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:3280360/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:3118240/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:3116921/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:2362766/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:2469610/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:836434/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:818506/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1174257/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1778072/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:3338295/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:3320443/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1651701/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1499171/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:2343771/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:2418869/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:30212/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:904102/68‑1 (MQ=255) gatgaGTCCAAGTCTGTTAAAGAAAAAATT‑‑GCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:353141/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:3296103/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:3080011/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:3406705/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:2924160/68‑1 (MQ=255) aGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:2944015/47‑1 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACTTCATATTCACAGTTGCTTACTGAgtgt > 1:3119699/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:1048146/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:112057/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:1809061/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:2932170/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:263826/1‑68 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:1369002/1‑69 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:497549/1‑69 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:1065624/1‑69 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:639506/1‑68 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:670743/1‑68 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:2465143/1‑68 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGATACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:3387706/69‑1 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:1631240/69‑1 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:2687939/69‑1 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:1061208/69‑1 (MQ=255) | AATATCGCCCATGAGTGGACACTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCA > minE/2582339‑2582466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |