| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,582,428 | G→T | 39.6% | E183D (GAG→GAT) ‡ | gadX → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,582,428 | 0 | G | T | 39.6% | 121.3 / 134.6 | 159 | E183D (GAG→GAT) ‡ | gadX | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (37/59); new base T (35/28); total (72/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
Only 100 of 161 total aligned reads displayed.
AGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATG > minE/2582362‑2582495 | aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAgaga > 1:2441034/1‑68 (MQ=255)aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAc > 1:2041802/1‑69 (MQ=255)aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAc > 1:178768/1‑69 (MQ=255)aGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAc > 1:3072214/1‑69 (MQ=255) cGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca < 1:2700881/69‑1 (MQ=255) gAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatcat < 1:9394/69‑1 (MQ=255) gAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca < 1:902800/68‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:3150758/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:1449535/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:1806884/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:409189/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:1065568/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:980247/69‑1 (MQ=255) tCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:1853104/1‑69 (MQ=255) tCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:2089172/1‑69 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:3219800/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:1727380/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:467565/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:780311/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:908036/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:28303/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:188726/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2087421/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:2934637/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2611066/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2659464/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2766711/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:1694617/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:839444/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1314138/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:3415282/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:3085261/1‑67 (MQ=255) gCCACCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:300261/1‑63 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:3116921/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:836434/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:2362766/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:2469610/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:2418869/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1174257/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:3324427/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:2343771/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:215502/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:2924160/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:3296103/68‑1 (MQ=255) aGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:2944015/47‑1 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACTTCATATTCACAGTTGCTTACTGAgtgt > 1:3119699/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:3018889/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:1048146/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:2640854/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:1809061/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:112057/1‑68 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:1065624/1‑69 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:1369002/1‑69 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:497549/1‑69 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAgtg > 1:3276379/1‑62 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:670743/1‑68 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:639506/1‑68 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:786002/1‑68 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGATACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:3387706/69‑1 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:1631240/69‑1 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:3435216/69‑1 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:82694/1‑69 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:886028/1‑69 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:138586/1‑69 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:314828/1‑69 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGc > 1:3107855/1‑69 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGc > 1:1997635/1‑69 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGc > 1:1090697/1‑69 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:736140/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2769289/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:812313/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:1092441/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:961632/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:1009683/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2880830/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2425482/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2238066/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1392105/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1394660/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1095540/69‑1 (MQ=255) cgAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCGACTTATTGt < 1:601097/69‑1 (MQ=255) cgAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGt < 1:3372135/69‑1 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGAt > 1:3064514/1‑44 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2827488/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2845648/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2607370/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2949311/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2188231/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:799209/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1859140/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1130849/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:165841/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3181138/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3185064/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:551612/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1538642/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1346411/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3250903/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3304472/1‑54 (MQ=255) agaaggGATGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:7542/1‑52 (MQ=255) | AGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATG > minE/2582362‑2582495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |