Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,582,431 | A→G | 44.4% | T184T (ACA→ACG) | gadX → | DNA‑binding transcriptional dual regulator |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,582,431 | 0 | A | G | 44.4% | 30.1 / 115.8 | 141 | T184T (ACA→ACG) | gadX | DNA‑binding transcriptional dual regulator |
Reads supporting (aligned to +/- strand): ref base A (26/52); new base G (35/27); total (62/79) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.79e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
Only 100 of 154 total aligned reads displayed.
CGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATG > minE/2582366‑2582495 | cGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca < 1:2424444/69‑1 (MQ=255) cGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca < 1:2700881/69‑1 (MQ=255) gAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatcat < 1:1389000/69‑1 (MQ=255) gAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatcat < 1:9394/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:980247/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:409189/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:3150758/69‑1 (MQ=255) aaTCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:1065568/69‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:780311/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:908036/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:3227288/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:467565/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:1727380/1‑63 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:2934637/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:3085261/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2766711/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:28303/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:2087421/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:188726/1‑67 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1814612/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:3415282/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1163261/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1314138/67‑1 (MQ=255) gCCAGCGAGCTGTTGATGAGTCCAAGTCTCTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt < 1:1959453/67‑1 (MQ=255) gCCAGCGAGCTATTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATAtt > 1:765152/1‑67 (MQ=255) gCCACCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGAcatca > 1:300261/1‑63 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:836434/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:3280360/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:1356107/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:3116921/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:3118240/68‑1 (MQ=255) cAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:2362766/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1651701/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:904102/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:818506/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:30212/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:2418869/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:2343771/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:215502/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1499171/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:3320443/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:1778072/68‑1 (MQ=255) aGCGAATTGTTGATGAGTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTcaca < 1:3324427/68‑1 (MQ=255) gatgaGTCCAAGTCTGTTAAAGAAAAAATT‑‑GCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:353141/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:3296103/68‑1 (MQ=255) tgaGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:2924160/68‑1 (MQ=255) aGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCata < 1:2944015/47‑1 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:1048146/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:1809061/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:2640854/1‑68 (MQ=255) gTCCAAGCCTGTTAAAGAAAAAATTGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAgtgt > 1:263826/1‑68 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:1065624/1‑69 (MQ=255) cAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGaa > 1:497549/1‑69 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:2465143/1‑68 (MQ=255) aGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAAt > 1:639506/1‑68 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGATACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:3387706/69‑1 (MQ=255) gTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGc < 1:2687939/69‑1 (MQ=255) tCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCa < 1:1091868/69‑1 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTcac < 1:740008/43‑1 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:886028/1‑69 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:874854/1‑69 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:138586/1‑69 (MQ=255) cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:1202394/1‑69 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAg < 1:3099058/52‑1 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGc > 1:1090697/1‑69 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:736140/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:594515/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:1009683/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:812313/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:961632/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2238066/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2880830/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2769289/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:3136936/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:3163779/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1394660/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:2138495/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1100882/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1095540/69‑1 (MQ=255) cgAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGt < 1:3372135/69‑1 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGAt > 1:3064514/1‑44 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2607370/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2539672/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:833768/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2827488/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2949311/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2845648/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1859140/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:797712/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1130849/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2188231/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3185064/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:551612/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3186527/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1393679/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1538642/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3250903/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:165841/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACAGAGTGTAGGATGCAGCGTGCt > 1:65563/1‑54 (MQ=255) agaCATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATg < 1:2048596/69‑1 (MQ=255) | CGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATG > minE/2582366‑2582495 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |