| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,582,467 | A→G | 51.0% | Q196Q (CAA→CAG) | gadX → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,582,467 | 0 | A | G | 51.0% | 3.4 / 93.7 | 104 | Q196Q (CAA→CAG) | gadX | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (21/30); new base G (27/26); total (48/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCTGTGGA > minE/2582399‑2582530 | cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:314828/1‑69 (MQ=255)cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:138586/1‑69 (MQ=255)cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:82694/1‑69 (MQ=255)cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:874854/1‑69 (MQ=255)cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:1202394/1‑69 (MQ=255)cTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCaa > 1:886028/1‑69 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGc > 1:3107855/1‑69 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGc > 1:1997635/1‑69 (MQ=255) aaaGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGc > 1:1090697/1‑69 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:3163779/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:1092441/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2769289/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2238066/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2880830/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:961632/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2877622/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:2425482/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:736140/69‑1 (MQ=255) tGCGCGAAGAAGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:1009683/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1095540/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1394660/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1392105/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:915022/69‑1 (MQ=255) tGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTa < 1:1100882/69‑1 (MQ=255) cgAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCGACTTATTGt < 1:601097/69‑1 (MQ=255) cgAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGt < 1:3372135/69‑1 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2827488/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2949311/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:551612/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2607370/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3181138/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3185064/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3186527/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3250903/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:3304472/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:445362/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2845648/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:797712/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:799209/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:833768/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:974132/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2539672/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1859140/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2184999/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1069935/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1130849/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1346411/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:165841/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1393679/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:2188231/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:1538642/1‑54 (MQ=255) gaagaaGGTACGTCATATTCACAGTTGCTTACAGAGTGTAGGATGCAGCGTGCt > 1:65563/1‑54 (MQ=255) agaaggGATGTCATATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCt > 1:7542/1‑52 (MQ=255) agaCATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATg < 1:2048596/69‑1 (MQ=255) aCATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGt < 1:2415400/59‑1 (MQ=255) cgtcatATTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTa < 1:1035248/52‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:1496901/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:573972/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:1357719/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:937589/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:3366496/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:2629520/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:2471229/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:2384279/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:2797691/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:2488976/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGGATGCAGCGTGCTTTACAACTTATTGTTATATATGGGGTTTc < 1:2732758/69‑1 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTc > 1:3184646/1‑69 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTc > 1:2804168/1‑69 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTc > 1:1755911/1‑69 (MQ=255) atatTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTc > 1:1746125/1‑69 (MQ=255) ttCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCaa > 1:315050/1‑68 (MQ=255) ttCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCaa > 1:214687/1‑68 (MQ=255) tCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCa < 1:3257383/66‑1 (MQ=255) tCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCa < 1:3024973/66‑1 (MQ=255) tCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCa < 1:269722/66‑1 (MQ=255) gTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAgag < 1:2408986/69‑3 (MQ=255) gTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCg < 1:1966393/69‑1 (MQ=255) tACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGc < 1:805023/69‑1 (MQ=255) gAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTa < 1:1710618/69‑1 (MQ=255) gAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTa < 1:366480/69‑1 (MQ=255) gAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTa < 1:425087/69‑1 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATcc > 1:693137/1‑67 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:922724/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:1332483/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:817678/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:1974799/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:2850758/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:787272/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:574935/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:3085785/1‑69 (MQ=255) tAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtg > 1:3123018/1‑69 (MQ=255) gAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtgtg < 1:771048/69‑1 (MQ=255) gAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtgtg < 1:2932429/69‑1 (MQ=255) gAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtgtg < 1:1708883/69‑1 (MQ=255) gAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtgtg < 1:34884/69‑1 (MQ=255) gAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtgtg < 1:3256873/69‑1 (MQ=255) gAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtgtg < 1:1048098/69‑1 (MQ=255) gAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCtgtg < 1:2688452/69‑1 (MQ=255) aTGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCTGTGGa < 1:1505549/69‑1 (MQ=255) | CTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTCACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAGTATCCTGTGGA > minE/2582399‑2582530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |