| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,583,233 | T→A | 44.8% | D54E (GAT→GAA) | gadW → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,583,233 | 0 | T | A | 44.8% | 21.5 / 94.6 | 87 | D54E (GAT→GAA) | gadW | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (26/22); new base A (9/30); total (35/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AATTTGGCAGACGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTA > minE/2583177‑2583293 | aaTTTGGCAGTCGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAg < 1:883725/67‑1 (MQ=255) cGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCAttact > 1:4406/1‑68 (MQ=255) ttGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACaaaa < 1:2752479/63‑1 (MQ=255) ttGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACaaaa < 1:777230/63‑1 (MQ=255) ttGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACaaaa < 1:2348350/63‑1 (MQ=255) ttGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACaaaa < 1:2261980/63‑1 (MQ=255) ttGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACaaaa < 1:1417860/63‑1 (MQ=255) tGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACaaaa < 1:475061/62‑1 (MQ=255) ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:895557/68‑1 (MQ=255) ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:2950894/68‑1 (MQ=255) ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:3170881/68‑1 (MQ=255) ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1003081/68‑1 (MQ=255) ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1647067/68‑1 (MQ=255) ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1200908/68‑1 (MQ=255) ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1023857/68‑1 (MQ=255) cGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:2946895/67‑1 (MQ=255) tACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACaaaa < 1:1912707/51‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTAc > 1:522933/1‑63 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:3123233/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2904200/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa < 1:1024354/69‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:3007590/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa < 1:2519817/69‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2477735/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2366186/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1072578/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2281343/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:3252510/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2255862/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa < 1:42414/69‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1997433/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1855374/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:60473/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1378476/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:138697/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:489739/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:721474/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1852881/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1131471/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAAAATTCGTCAGCTACCACGCa > 1:475726/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCaa > 1:584988/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCaa > 1:866624/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCaa > 1:1638381/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1797541/1‑68 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2757274/1‑68 (MQ=255) cTGGATATCGAAGAGCTGAGAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1413155/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTatga < 1:1699301/69‑4 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:3368738/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:3155873/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:66838/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:710543/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:3224348/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:3017990/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:552435/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2424771/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:753898/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2277193/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1224763/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1221059/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1518334/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1639138/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1821443/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1216548/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1914824/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1127931/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1125905/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2108150/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2310360/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2593781/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1068111/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1067834/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1020961/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2897359/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1011061/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2945550/69‑2 (MQ=255) tatCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:2910683/67‑1 (MQ=255) tatCGATGAGCTAACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:2943612/67‑1 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:188801/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:1171036/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:393511/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:3413571/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:2609820/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:3145222/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:2774271/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:2980762/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCATCAGTTGCCACGCAATTTAGGAttg > 1:141004/1‑66 (MQ=255) atCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:1261081/66‑1 (MQ=255) | AATTTGGCAGACGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTA > minE/2583177‑2583293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |