| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,583,273 | C→T | 35.0% | L68L (CTA→TTA) ‡ | gadW → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,583,273 | 0 | C | T | 35.0% | 87.6 / 64.9 | 114 | L68L (CTA→TTA) ‡ | gadW | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (26/48); new base T (9/31); total (35/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
CCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCAGTCAC > minE/2583207‑2583327 | ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:2950894/68‑1 (MQ=255)ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1647067/68‑1 (MQ=255)ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1003081/68‑1 (MQ=255)ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1200908/68‑1 (MQ=255)ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:3170881/68‑1 (MQ=255)ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:1023857/68‑1 (MQ=255)ccGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCt < 1:895557/68‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTAc > 1:522933/1‑63 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:489739/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa < 1:42414/69‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1852881/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1855374/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1997433/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2281343/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2366186/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2477735/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa < 1:2519817/69‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2904200/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:2255862/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:3007590/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:60473/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:3123233/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:3252510/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1131471/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1072578/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1378476/1‑69 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa < 1:1024354/69‑1 (MQ=255) cgcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:138697/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCaa > 1:866624/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCaa > 1:584988/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCaa > 1:1638381/1‑69 (MQ=255) gcgACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCa > 1:1797541/1‑68 (MQ=255) cTGGATATCGAAGAGCTGAGAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1413155/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTatga < 1:1699301/69‑4 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1224763/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1221059/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1011061/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2593781/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:66838/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2897359/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:710543/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2945550/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1127931/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1125905/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:3017990/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1068111/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:3224348/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:552435/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1020961/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2310360/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1518334/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1914824/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2108150/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:2277193/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:753898/69‑2 (MQ=255) cTGGATATCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1821443/69‑2 (MQ=255) tatCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:2910683/67‑1 (MQ=255) tatCGATGAGCTAACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:2943612/67‑1 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:2774271/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:2980762/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:3145222/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:3413571/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:2609820/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:393511/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAGGAttg > 1:1171036/1‑66 (MQ=255) tatCGAAGAGCTGACAGTTTGCCATTACTTACAAAATATTCATCAGTTGCCACGCAATTTAGGAttg > 1:141004/1‑66 (MQ=255) atCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:1261081/66‑1 (MQ=255) aGCTGACAGTTTGCCATTACTTACAAGATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAg < 1:894418/69‑1 (MQ=255) aGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAg < 1:318750/69‑1 (MQ=255) aGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAg < 1:1032317/69‑1 (MQ=255) aGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAg < 1:2003529/69‑1 (MQ=255) cTGACAGTTTGCCATTACTTACAAAATATTCGTCAGTTGCCACGCAATTTAgga < 1:1503421/54‑2 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:3341402/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:1566431/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:2514855/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:3422240/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:2812237/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:1657699/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:1066130/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTa < 1:1926495/54‑1 (MQ=255) aCAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGt > 1:1297222/1‑69 (MQ=255) ttGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGtt < 1:334465/69‑1 (MQ=255) ttGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGtt < 1:376690/69‑1 (MQ=255) ttGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGtt < 1:2525427/69‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:2446478/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:840428/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:851699/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:421648/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:3010231/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:239135/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:2204478/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:208992/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:1307065/68‑1 (MQ=255) ttacAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:1016615/68‑1 (MQ=255) acAAAATATTCGTCAGCTACCACGCAATTTATGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:2684592/66‑1 (MQ=255) acAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:474278/66‑1 (MQ=255) acAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:2267206/66‑1 (MQ=255) acAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:1432574/66‑1 (MQ=255) acAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCa < 1:947580/66‑1 (MQ=255) aaaTATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCAGTCAc < 1:3252562/68‑1 (MQ=255) | CCGGATACGCGACGACTGGATATCGATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACACAGCAAAGACCGTTTGTTAATTAACCAGTCAC > minE/2583207‑2583327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |