| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,463 | T→C | 45.6% | K938K (AAA→AAG) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,463 | 0 | T | C | 45.6% | 17.9 / 107.2 | 125 | K938K (AAA→AAG) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (41/27); new base C (25/32); total (66/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 126 total aligned reads displayed.
TCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGAAGTAGACGTCATTACTTAAGCCG > minE/2584402‑2584526 | tCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTTGCg > 1:2394299/1‑68 (MQ=255)tCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCg > 1:2421951/1‑68 (MQ=255)tCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCg > 1:153168/1‑68 (MQ=255) ggCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGtttt > 1:1907931/1‑61 (MQ=255) ggCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGaa > 1:627169/1‑69 (MQ=255) ggCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGaa > 1:1860496/1‑69 (MQ=255) ggCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGaa > 1:3409692/1‑69 (MQ=255) ggCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGaa > 1:2556190/1‑69 (MQ=255) cccTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGa < 1:550845/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATtgtggt < 1:546047/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:1197770/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:2762904/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:2217468/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:2661412/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:1840395/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:1677203/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:560552/68‑1 (MQ=255) ttctGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATggtggt < 1:91318/68‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAg > 1:2251621/1‑68 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAg > 1:2168353/1‑68 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAg > 1:2299987/1‑68 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2299275/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2266911/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2750083/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:856274/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2167620/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2159306/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2130785/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2909145/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2956137/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:655674/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:3061234/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2471164/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:146149/67‑1 (MQ=255) tGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:548092/67‑1 (MQ=255) gCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:2639836/66‑1 (MQ=255) gCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCa < 1:95315/66‑1 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:2825106/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:2980840/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:1195314/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca < 1:595148/69‑1 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:608970/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:2423189/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca < 1:866908/69‑1 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:869598/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:1554235/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:1929826/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca < 1:1217988/69‑1 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca < 1:122783/69‑1 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca < 1:1779344/69‑1 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:2359962/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:1759061/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacca > 1:1868152/1‑69 (MQ=255) atcTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaacc > 1:1363941/1‑68 (MQ=255) ctcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaa < 1:3095650/68‑1 (MQ=255) ctcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaa < 1:1993092/68‑1 (MQ=255) ctcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaa < 1:2773/68‑1 (MQ=255) ctcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaa < 1:2302886/68‑1 (MQ=255) ctcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaa < 1:804742/68‑1 (MQ=255) ctcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaa < 1:2355955/68‑1 (MQ=255) tcAACTGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2734709/1‑60 (MQ=39) tcAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaacc > 1:3069032/1‑69 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaacc > 1:876545/1‑69 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaacc > 1:2365622/1‑69 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAaaccaacc > 1:2467488/1‑69 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAg > 1:393480/1‑44 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:674789/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:1686729/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:1623004/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:1434477/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:1340761/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:1113702/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:674909/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:768270/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:1233880/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2369148/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:3298815/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2546726/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2525836/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:251114/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2218104/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2796744/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2072670/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2898509/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2034996/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:3209740/1‑60 (MQ=255) tcAACGGCAAATTCGACAATCAGGATGGCGTTCTTGGCGGAAAGCCCGATGGTGGTCAGc > 1:2497296/1‑60 (MQ=255) cGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTggg < 1:180032/69‑2 (MQ=255) cGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGa < 1:3037158/69‑1 (MQ=255) cGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGa < 1:2821466/69‑1 (MQ=255) cGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGa < 1:361462/69‑1 (MQ=255) cGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGa < 1:2732298/69‑1 (MQ=255) cGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGa < 1:704586/69‑1 (MQ=255) cGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGa < 1:1238537/69‑1 (MQ=255) cGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGAAGTAGACGt < 1:2426115/69‑1 (MQ=255) cAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGAAGTAGACGTCAt < 1:3338610/69‑1 (MQ=255) cAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGAAGTAGACGTCATTAc > 1:2245525/1‑68 (MQ=255) gCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGAAGTAGACGTCATTACTTAAGCcg > 1:2649697/1‑69 (MQ=255) gCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGAAGTAGACGTCATTACTTAAGCcg > 1:3252173/1‑69 (MQ=255) gCGTTCTTGGCGGAAAGCCCGATGGTGGGCAGCAaaccaa < 1:2640285/40‑1 (MQ=38) | TCGGCGTTTTCCCTTCTTTCTGCATCATCTCAACGGCAAATTCGACAATCAGGATGGCGTTTTTGGCGGAAAGCCCGATGGTGGTCAGCAAACCAACCTGGAAGTAGACGTCATTACTTAAGCCG > minE/2584402‑2584526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |