| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,697 | T→C | 40.8% | L860L (CTA→CTG) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,697 | 0 | T | C | 40.8% | 16.6 / 32.8 | 49 | L860L (CTA→CTG) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (16/13); new base C (6/14); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01 | |||||||||||
GAAATCGCATACAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCTGCCATAAATTTCATG > minE/2584644‑2584763 | gAAATCGCATACAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATg > 1:432159/1‑67 (MQ=255) aaaTCGCATACAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGt > 1:1632075/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:1028250/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:260839/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:2528871/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:2833152/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:2083813/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:2961702/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:3312102/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:1585927/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:333627/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:1338621/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:1334671/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:3356509/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGcc > 1:349801/1‑69 (MQ=255) cAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGc > 1:403602/1‑68 (MQ=255) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:3315649/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:3243130/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:3091190/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:2971150/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:3354596/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:290801/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:2820601/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:1036138/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:2769439/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:2735108/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:1037858/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:1083554/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:2279355/46‑1 (MQ=38) gggAGCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTcc < 1:2343125/46‑1 (MQ=38) gCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGt > 1:1975885/1‑69 (MQ=255) gCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGt > 1:908254/1‑69 (MQ=255) gCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGt > 1:1547217/1‑69 (MQ=255) gCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGt > 1:1476672/1‑69 (MQ=255) gCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGt > 1:1233600/1‑69 (MQ=255) gCCTGGTTTGAGGATAACGCTTCCTGATACGACAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGt > 1:2775118/1‑69 (MQ=255) ttGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGc < 1:2245450/67‑1 (MQ=255) ttGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGc < 1:2949164/67‑1 (MQ=255) ttGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGc < 1:1996187/67‑1 (MQ=255) tGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGAc < 1:2885577/69‑1 (MQ=255) tGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGAc < 1:1149449/69‑1 (MQ=255) aaCGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCt < 1:1061559/69‑1 (MQ=255) aCGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCt < 1:2743779/68‑1 (MQ=255) aCGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCt < 1:91989/68‑1 (MQ=255) aCGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCt < 1:989998/68‑1 (MQ=255) aCGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCt < 1:67913/68‑1 (MQ=255) aCGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCt < 1:1715027/68‑1 (MQ=255) aCGCTTCCTGATACGATAGTCCGGGCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCt < 1:191224/68‑1 (MQ=255) aTAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCTGCCATAAATTTCATg < 1:1930687/68‑1 (MQ=255) | GAAATCGCATACAGCGCAGGAGCCTGATTTGAGGATAACGCTTCCTGATACGATAGTCCGGTCCATGAGTAGCCGACGCCTGCCGGAAGTTTAGCGACCAGGTCTGCCATAAATTTCATG > minE/2584644‑2584763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |