| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,585,609 | G→A | 70.3% | R556R (CGC→CGT) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,585,609 | 0 | G | A | 70.3% | 50.4 / 24.5 | 57 | R556R (CGC→CGT) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (5/12); new base A (38/2); total (43/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GTAACTGCGCGGTGGTCATAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCGGTACAA > minE/2585548‑2585675 | gTAACTGCGCGGTGGTCATAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTGCGCAgg > 1:2087672/1‑68 (MQ=255)gTAACTGCGCGGTGGTCATAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGa < 1:1163828/69‑1 (MQ=255)gTAACTGCGCGGTGGTCATAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGa < 1:1580342/69‑1 (MQ=255) gtCATAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTGCGCAGGAACAGCACCGCCATc > 1:2744222/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATccc > 1:3309798/1‑68 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATccc > 1:2160372/1‑68 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:2570255/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:98254/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:326180/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:2630476/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:2498242/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:2046614/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:1332036/1‑69 (MQ=255) aTAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:1027114/1‑69 (MQ=255) aTAAATACCCACTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTACGCAGGAACAGCACCGCCATCCCg > 1:2369050/1‑69 (MQ=255) cttctGGTAAGAAAGAGGTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAg < 1:2964322/68‑1 (MQ=255) cttctGGTAAGAAAGAGGTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAg < 1:216251/68‑1 (MQ=255) cttctGGTAAGAAAGAGGTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAg < 1:2406229/68‑1 (MQ=255) ttctGGTAAGAAAGAGGTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAg < 1:2536337/67‑1 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATcagc > 1:3193975/1‑46 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2838993/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2787287/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2876581/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2983812/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:305491/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:3061925/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:3255138/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:1172131/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:3289219/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:1123516/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:3348535/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:631723/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:741655/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:977820/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:1958799/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:1992205/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:1955947/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2229052/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2312612/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2386030/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2407020/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:1705784/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:1005834/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2589343/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2718504/1‑69 (MQ=255) ggTCGGCGTACGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2729061/1‑69 (MQ=255) gTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:2631266/1‑68 (MQ=255) gTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:813466/1‑68 (MQ=255) gTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGAcc > 1:960808/1‑68 (MQ=255) tCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCgg < 1:1517685/69‑1 (MQ=255) tCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCgg < 1:615323/69‑1 (MQ=255) tCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCgg < 1:20693/69‑1 (MQ=255) tCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACAACCATGTAGCGACCgg < 1:3141093/69‑1 (MQ=255) tCGGCGTGCGCAGGAACAGCACCG‑CATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCgg < 1:2281180/68‑1 (MQ=255) gTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCGGTACaa < 1:180645/69‑1 (MQ=255) gTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCGGTACaa < 1:2060594/69‑1 (MQ=255) tGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCGGTACaa < 1:433800/68‑1 (MQ=255) | GTAACTGCGCGGTGGTCATAAATACCCCCTGATCCTCTTCTGGTAAGAAAGAGGTCGGCGTGCGCAGGAACAGCACCGCCATCCCGGCGCAAATCAGCAGGTAGACCACCATGTAGCGACCGGTACAA > minE/2585548‑2585675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |