| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,227 | T→G | 77.7% | L350L (CTA→CTC) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,227 | 0 | T | G | 77.7% | 115.0 / 23.7 | 67 | L350L (CTA→CTC) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (7/8); new base G (15/37); total (22/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAATT > minE/2586161‑2586275 | tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2727892/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:1862465/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:1990729/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2254348/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2283692/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2823665/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2910425/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2949683/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:1441596/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:3418716/69‑1 (MQ=255)tACGGCAATCGTCGGGATGATTGTGGCGCGGAAATCCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2703085/69‑1 (MQ=25) aCGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:434355/68‑1 (MQ=255) aCGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:1872878/68‑1 (MQ=255) aCGGCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2124525/68‑1 (MQ=255) aCGGCAATCGTCGGGAGGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGACGACGAg < 1:2203873/68‑1 (MQ=25) tcAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:1798888/68‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:797271/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:1946519/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:747324/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:208474/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:2096483/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:605808/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:477549/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:1069155/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:3126074/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:1663899/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:1414165/69‑1 (MQ=255) gCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGgat > 1:398961/1‑68 (MQ=255) gCAATCGTCGGGAAGATTGTGGCGCGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:1704617/69‑1 (MQ=255) cgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGc > 1:1774417/1‑69 (MQ=255) cgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGc > 1:2984725/1‑69 (MQ=255) cgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGc > 1:1341704/1‑69 (MQ=255) cgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGc > 1:1331141/1‑69 (MQ=255) cgtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGc > 1:755554/1‑69 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACt > 1:3196166/1‑57 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACt > 1:174583/1‑57 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACt > 1:3150062/1‑57 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACt > 1:1523261/1‑57 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACt > 1:2786893/1‑57 (MQ=255) gtcgGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACt > 1:1900434/1‑57 (MQ=255) gtgGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGgat > 1:179641/1‑50 (MQ=255) gcGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGaa < 1:2921638/68‑1 (MQ=18) gcGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGaa < 1:409547/68‑1 (MQ=18) gcGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGaa < 1:2735621/68‑1 (MQ=18) gcGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGaa < 1:1698671/68‑1 (MQ=18) gcGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGaa < 1:2458647/68‑1 (MQ=18) gAAATTCTGCAAAAACAGATACATGACCTGGAAGACGAGgatg < 1:1825886/43‑1 (MQ=38) gAAATTCTGCAAAAACAGATACATGACCAGGAAGACGAg < 1:2217260/39‑1 (MQ=255) tCTGCAAAAACAGATACATGACCAGGAAGACGAGgatg < 1:696259/38‑1 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATATcc > 1:3341357/1‑37 (MQ=17) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAg > 1:521153/1‑68 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAg > 1:3070/1‑68 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1332896/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1975862/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:711075/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1792700/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1143074/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1720448/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1705871/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:750595/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1058496/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:2530450/1‑69 (MQ=255) aaaCAGATACATGACCAGGAAGACGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTACTGAATAGa > 1:2648902/1‑69 (MQ=255) aaaCAGATACATGACCAGAAAGGCGAGGATGATAGCCTCAACCAGCGTTTTGAAAACTTCCTGAATAGa > 1:1523810/1‑69 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:19643/69‑1 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:608564/69‑1 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:615388/69‑1 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:2021312/69‑1 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:3430073/69‑1 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:1789365/69‑1 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:2438821/69‑1 (MQ=255) aGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAAtt < 1:1848019/69‑1 (MQ=255) gATACATGACCAGGAAGACGAGGATGATAGCCTCAAGCAGCGTTTTGaa < 1:1333247/49‑1 (MQ=255) | TACGGCAATCGTCGGGATGATTGTGGCACGGAAATTCTGCAAAAACAGATACATGACCAGGAAGACTAGGATGATAGCCTCAACCAGTGTTTTGAAAACTTCCTGAATAGAAATT > minE/2586161‑2586275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |