| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,587,031 | A→G | 24.9% | S82S (AGT→AGC) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,587,031 | 0 | A | G | 24.9% | 188.1 / 59.2 | 156 | S82S (AGT→AGC) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (30/87); new base G (20/19); total (50/106) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
Only 100 of 158 total aligned reads displayed.
CGATATCAGGAGATGTCCCAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCAATCACCTGAGTGACCGA > minE/2586970‑2587099 | gagaTGTCCCAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:3091380/68‑1 (MQ=255) gagaTGTCCCAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:2320705/68‑1 (MQ=255) gagaTGTCCCAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:895570/68‑1 (MQ=255) agaTGTCCCAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:1053603/69‑1 (MQ=255) gaTGTCCCAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTAc > 1:1583862/1‑69 (MQ=255) ccAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAg < 1:277079/68‑1 (MQ=255) ccAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAg < 1:3037747/68‑1 (MQ=255) ccAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAg < 1:298260/68‑1 (MQ=255) ccAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAg < 1:2120225/68‑1 (MQ=255) ccAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAg < 1:871118/68‑1 (MQ=255) ccAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAg < 1:91712/68‑1 (MQ=255) ccAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAg < 1:1817259/68‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGTTAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:3383741/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGTCATGTa < 1:436306/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:339990/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:2689060/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:3068563/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:1852549/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:2521624/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:382441/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:2072937/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:457941/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:718097/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:922226/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTa < 1:2263492/60‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGc < 1:94298/68‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGc < 1:2108714/68‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGc < 1:2568968/68‑1 (MQ=255) aGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGc < 1:682374/68‑1 (MQ=255) ctcGAAGGTCAGTGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:2152677/55‑1 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg > 1:2637518/1‑55 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:2714482/55‑1 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:3097449/55‑1 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:3368878/55‑1 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg > 1:403965/1‑55 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg > 1:647017/1‑55 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg > 1:2276859/1‑55 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg > 1:101612/1‑55 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:1014044/55‑1 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg > 1:1370278/1‑55 (MQ=255) ctcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATg < 1:1128787/55‑1 (MQ=255) tcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCa > 1:2020164/1‑69 (MQ=255) tcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCa > 1:2773887/1‑69 (MQ=255) tcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCa > 1:1559539/1‑69 (MQ=255) tcGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCa > 1:79716/1‑69 (MQ=255) tCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGt < 1:2706070/37‑1 (MQ=255) agagTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTc < 1:628735/69‑1 (MQ=255) agagTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTc < 1:2579829/69‑1 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:1278252/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:543466/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:524659/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:2134557/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:1441001/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:3332510/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:3088369/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:1659866/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:2947261/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:1056403/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:2243889/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAttt > 1:1996907/1‑65 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAtt > 1:291385/1‑64 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATAtt > 1:1540385/1‑64 (MQ=255) agGCATTACCTGCCGCATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGACCATTCATAttt > 1:484122/1‑65 (MQ=38) ggCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTc < 1:2134307/69‑1 (MQ=255) ggCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTc < 1:2282790/69‑1 (MQ=255) ggCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTc < 1:1171372/69‑1 (MQ=255) ggCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTc < 1:3256954/69‑1 (MQ=255) ggCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTc < 1:257788/69‑1 (MQ=255) ggCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTc < 1:1300861/69‑1 (MQ=255) gCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCTATCAAGCCCATTCATATTTTGCTCa < 1:574417/69‑1 (MQ=255) gCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCa < 1:2737655/69‑1 (MQ=255) gCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCa < 1:3139567/69‑1 (MQ=255) ttGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAgac < 1:2412770/49‑3 (MQ=255) ttGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGcc < 1:1729256/49‑1 (MQ=255) ttGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGcc < 1:899360/49‑1 (MQ=255) ttGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGcc < 1:1210016/49‑1 (MQ=255) ttGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGcc < 1:545236/49‑1 (MQ=255) ttGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGcc < 1:3246802/49‑1 (MQ=255) gCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCAATCa > 1:526911/1‑68 (MQ=255) gCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCAATCAc > 1:232936/1‑69 (MQ=255) gCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCAATCAc > 1:1502761/1‑69 (MQ=255) gCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCAATCAc > 1:1233101/1‑69 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:3140211/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1777399/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1789964/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1123396/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1808537/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1905538/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:2936883/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:72746/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1519354/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:3414748/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:2425607/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:2489664/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1471923/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1925533/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1322604/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:1333064/54‑1 (MQ=255) cgcATCGCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTg < 1:2928648/54‑1 (MQ=255) aCTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCAATCACCTGAGTGAcc > 1:2370103/1‑67 (MQ=255) | CGATATCAGGAGATGTCCCAGTCTCGAAGGTCAGAGTGATAGAGGCATTGCCCGCCGCATCACTGGTTGAAGACATGTACATCAGGCCATCAAGCCCATTCATATTTTGCTCAATCACCTGAGTGACCGA > minE/2586970‑2587099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |