| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,587,208 | A→G | 22.6% | G23G (GGT→GGC) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,587,208 | 0 | A | G | 22.6% | 157.0 / 38.0 | 119 | G23G (GGT→GGC) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (16/76); new base G (10/17); total (26/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATA > minE/2587146‑2587267 | gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:1052495/69‑1 (MQ=255)gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:816955/69‑1 (MQ=255)gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:2154457/69‑1 (MQ=255)gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:1699072/69‑1 (MQ=255)gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:2396339/69‑1 (MQ=255)gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:163359/69‑1 (MQ=255)gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:1756781/69‑1 (MQ=255)gTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCa < 1:434532/69‑1 (MQ=255) aaTGTTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:1692398/69‑1 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCaa > 1:3169824/1‑68 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:2552770/69‑1 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg > 1:3338952/1‑69 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg > 1:1756412/1‑69 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg > 1:2562701/1‑69 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:3336128/69‑1 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:1832801/69‑1 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:2452526/69‑1 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg > 1:2110578/1‑69 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg > 1:2116682/1‑69 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg > 1:2376575/1‑69 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:1437098/69‑1 (MQ=255) aaTGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg > 1:513460/1‑69 (MQ=255) ggTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGcat < 1:1481198/69‑1 (MQ=255) gTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGcat > 1:2526830/1‑68 (MQ=255) gTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGcat < 1:732773/68‑1 (MQ=255) gTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGcat > 1:1188125/1‑68 (MQ=255) gTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGcat > 1:880318/1‑68 (MQ=255) tGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat < 1:2986101/69‑1 (MQ=255) tGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat < 1:1634151/69‑1 (MQ=255) tGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat < 1:1649179/69‑1 (MQ=255) tGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat < 1:580150/69‑1 (MQ=255) tGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat < 1:1825029/69‑1 (MQ=255) tGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat < 1:334464/69‑1 (MQ=255) ggCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat < 1:1800215/68‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCATACCACCTGCAAg < 1:755613/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:641362/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:2564471/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:511297/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:2880703/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:3409925/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:2902838/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:3409359/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:1027396/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:1373008/50‑1 (MQ=255) tGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:1460293/50‑1 (MQ=255) gATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATCATAATGGCAAGTAc < 1:3195246/67‑1 (MQ=255) gATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTa < 1:2852178/66‑1 (MQ=255) gATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTAc < 1:2278354/67‑1 (MQ=255) gATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTAc < 1:1826818/67‑1 (MQ=255) cTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAgg > 1:1852440/1‑68 (MQ=255) tGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAg < 1:262651/41‑1 (MQ=255) gcgcAACCGGTAAGTTCATGATCGCCAGACCACCTGCAA‑CATCATAATAATGGCAAGTACCCAGGCaaa < 1:2154769/69‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaaa < 1:2754178/68‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaaa < 1:2992573/68‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaaa < 1:254436/68‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaaa < 1:855295/68‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:430933/67‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:147311/67‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:1770969/67‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:923774/67‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:3176617/67‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:20979/67‑1 (MQ=255) cAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:2591188/67‑1 (MQ=255) ggTAAGTTCATGATCGCCAGACCACCTGCAAGCATCataat > 1:502742/1‑41 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:345685/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:1733845/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:2459207/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:1008256/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:1638263/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:3106149/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:1708900/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:1214469/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCg > 1:1437078/1‑68 (MQ=255) aaGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:2725146/59‑1 (MQ=255) aaGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:1805873/59‑1 (MQ=255) aaGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCaaa < 1:2735490/59‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:2105576/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:1379362/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:58615/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:79025/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:1099690/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:89422/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:2287017/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:458992/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:1820436/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:3336251/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:1686299/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:3181973/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:2996399/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:1729332/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:2960998/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAAcc < 1:2706523/56‑1 (MQ=255) tGATCGCCAGACCGCCTGCAAGCATCATAATAATGGCAAGTA‑‑CAGGCAAAAAcc < 1:1475236/54‑1 (MQ=255) gATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATaa < 1:1530378/68‑1 (MQ=255) gATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATaa < 1:1551633/68‑1 (MQ=255) gATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATaa < 1:1660912/68‑1 (MQ=255) gATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATaa < 1:661810/68‑1 (MQ=255) gATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATaa < 1:3283537/68‑1 (MQ=255) gATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATaa < 1:2593759/68‑1 (MQ=255) gATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATaa < 1:2296039/68‑1 (MQ=255) | GTAATGGTCGGTGGCGCAATCTGCGGATACTGCGCAACCGGTAAGTTCATGATCGCCAGACCACCTGCAAGCATCATAATAATGGCAAGTACCCAGGCAAAAACCGGGCGATCAATAAAATA > minE/2587146‑2587267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |