| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,587,673 | T→C | 58.5% | T262T (ACA→ACG) | mdtE ← | multidrug resistance efflux transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,587,673 | 0 | T | C | 58.5% | 25.9 / 51.3 | 73 | T262T (ACA→ACG) | mdtE | multidrug resistance efflux transporter |
| Reads supporting (aligned to +/- strand): ref base T (6/24); new base C (21/21); total (27/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
AAGTCACCATTTGGGTTGGGGAAAATCGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTA > minE/2587611‑2587740 | aaGTCACCATTTGGGTTGGGGAAAATCGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTgtcggg > 1:185266/1‑69 (MQ=255)aaGTCACCATTTGGGTTGGGGAAAATCGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTgtcggg > 1:1306720/1‑69 (MQ=255) cATTTGGGTTGGGGAAAATCGCCCGTAACGTCATGGAGCCCGTGGTTTCATCCACTGTCGGGTCGgag < 1:3000914/68‑1 (MQ=255) cATTTGGGTTGGGGAAAATCGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGgag < 1:417889/68‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCTTCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:1328335/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:866804/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:83754/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:811649/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:1206353/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:1926257/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:2011693/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTg < 1:910986/69‑1 (MQ=255) cGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGCTCGGAGAATTTCAGCGTGCCGGTCTg < 1:1570443/69‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:991902/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1873584/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:2415763/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:2804427/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:3229291/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:3355392/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:392807/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1008955/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:508156/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:693378/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:790973/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1793409/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1000994/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1317105/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1321097/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1324475/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:134816/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1376796/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1506368/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1841391/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACCGTCGGGTCGGAGAAtt < 1:1725323/48‑1 (MQ=255) gTAACGTCACGGAGCCCGTGGTTTCATCCACAGTCGGGTCGGAGAAtt < 1:3169605/48‑1 (MQ=255) gTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCg < 1:21138/68‑1 (MQ=255) gTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCg < 1:1204937/68‑1 (MQ=255) ggTTTCATCCACTGTCGGGTTGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2344097/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2138441/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:1418123/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:1430114/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:3178914/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2542163/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2596977/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCG‑TTACCATTTTCCAg < 1:1443334/68‑1 (MQ=255) gTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGa > 1:2702675/1‑69 (MQ=255) gTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGa > 1:2354922/1‑69 (MQ=255) gTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGa > 1:3129476/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGGCGTAACGTTTACCATTTTCCAGATTGAg > 1:1546154/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTcc > 1:1346458/1‑61 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGa > 1:3279920/1‑68 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGa > 1:3390262/1‑68 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:53074/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:667615/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:669185/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:1112156/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:790031/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:978791/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:3192152/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2248026/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2202871/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2995316/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2969939/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2836769/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2476623/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2407440/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2292017/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2264476/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCCGTCTg > 1:1685114/1‑39 (MQ=38) cACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg > 1:2013957/1‑60 (MQ=255) cACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTg < 1:3046563/69‑1 (MQ=255) cACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTg < 1:2057577/69‑1 (MQ=255) cTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTa < 1:757554/69‑1 (MQ=255) | AAGTCACCATTTGGGTTGGGGAAAATCGCCCGTAACGTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTA > minE/2587611‑2587740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |