| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,587,712 | G→A | 45.4% | R249R (CGC→CGT) | mdtE ← | multidrug resistance efflux transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,587,712 | 0 | G | A | 45.4% | 12.0 / 51.0 | 66 | R249R (CGC→CGT) | mdtE | multidrug resistance efflux transporter |
| Reads supporting (aligned to +/- strand): ref base G (8/28); new base A (20/10); total (28/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGATTTGCCCACTGGC > minE/2587647‑2587780 | gTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCg < 1:1204937/68‑1 (MQ=255)gTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCg < 1:21138/68‑1 (MQ=255) ggTTTCATCCACTGTCGGGTTGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2344097/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2542163/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2596977/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:1418123/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:1430114/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:2138441/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg < 1:3178914/69‑1 (MQ=255) ggTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCG‑TTACCATTTTCCAg < 1:1443334/68‑1 (MQ=255) gTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGa > 1:2354922/1‑69 (MQ=255) gTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGa > 1:2702675/1‑69 (MQ=255) gTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGa > 1:3129476/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGGCGTAACGTTTACCATTTTCCAGATTGAg > 1:1546154/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTcc > 1:1346458/1‑61 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGa > 1:3279920/1‑68 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGa > 1:3390262/1‑68 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:3192152/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2995316/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2969939/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:1112156/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2836769/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:53074/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:667615/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:669185/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2476623/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2407440/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:790031/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:978791/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2292017/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2264476/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2248026/1‑69 (MQ=255) aTCCACCGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAg > 1:2202871/1‑69 (MQ=255) cACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAg > 1:2013957/1‑60 (MQ=255) cACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTg < 1:3046563/69‑1 (MQ=255) cACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTg < 1:2057577/69‑1 (MQ=255) cTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTa < 1:757554/69‑1 (MQ=255) gtcggAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCg < 1:1912672/68‑1 (MQ=255) gtcggAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCg < 1:1848933/68‑1 (MQ=255) gtcggAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCg < 1:3132426/68‑1 (MQ=255) gtcggAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCg < 1:3009437/68‑1 (MQ=255) gtcggAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCg < 1:1377712/68‑1 (MQ=255) gtcggAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCg < 1:2920119/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:1823752/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:701585/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:339121/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:1340359/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:825021/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:88257/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:1326692/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:2291813/68‑1 (MQ=255) agaATTTCAGCGTGCCGGTCTGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:2472215/68‑1 (MQ=255) tttCAGCGTGCCGGTCTGGCTGTAGCTTTTACCATTTTCCAGATTGAGCTg < 1:1871559/51‑1 (MQ=255) tttCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCg < 1:1361297/59‑1 (MQ=255) ccGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAg > 1:538107/1‑38 (MQ=255) ccGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTg > 1:2015536/1‑69 (MQ=255) ccGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTg > 1:247945/1‑69 (MQ=255) ccGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTg > 1:2171403/1‑69 (MQ=255) ggTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGa < 1:66385/68‑1 (MQ=255) ggTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGa < 1:2295251/68‑1 (MQ=255) ggTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGa < 1:224992/68‑1 (MQ=255) ggTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGa < 1:3244596/68‑1 (MQ=255) ggTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGa < 1:3372011/68‑1 (MQ=255) ggTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGa < 1:3246172/68‑1 (MQ=255) tGGCCGTAACGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTg < 1:20886/48‑1 (MQ=255) tGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGATTTGcc < 1:294737/66‑1 (MQ=255) gCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGATTTGCCCACTGGc > 1:3181361/1‑69 (MQ=255) | GTCACGGAGCCCGTGGTTTCATCCACTGTCGGGTCGGAGAATTTCAGCGTGCCGGTCTGGCTGTAGCGTTTACCATTTTCCAGATTGAGCTGTACTGGCGTACTGCCCTGAACCTGTTTGATTTGCCCACTGGC > minE/2587647‑2587780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |