Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,588,911 C→T 16.3% S138S (TCG→TCA gadE ← DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,588,9110CT16.3% 173.9 / 18.5 103S138S (TCG→TCAgadEDNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (23/63);  new base T (8/9);  total (31/72)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 107 total aligned reads displayed.

TTAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGATTA  >  minE/2588844‑2588971
                                                                   |                                                            
ttAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGGGTCg                                                             >  1:2526367/1‑69 (MQ=255)
ttAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCg                                                             >  1:2008389/1‑69 (MQ=255)
ttAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCg                                                             >  1:1433840/1‑69 (MQ=255)
ttAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCg                                                             >  1:1440101/1‑69 (MQ=255)
ttAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCg                                                             >  1:925478/1‑69 (MQ=255)
ttAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCg                                                             >  1:1058316/1‑69 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:2025964/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:746878/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:2270225/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:562991/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:3360574/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:1540180/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:3134319/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:2570626/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:2834800/66‑1 (MQ=255)
    tttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGa                                                            <  1:1304467/66‑1 (MQ=255)
     ttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGt                                                         <  1:1836921/68‑1 (MQ=255)
     ttCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGt                                                         <  1:1220909/68‑1 (MQ=255)
      tCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGa                                                       <  1:1586551/69‑1 (MQ=255)
          cTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATAtt                                                   <  1:1445433/69‑1 (MQ=255)
          cTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATAtt                                                   <  1:66/69‑1 (MQ=255)
          cTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATAtt                                                   <  1:1215251/69‑1 (MQ=255)
          cTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATAtt                                                   <  1:1517379/69‑1 (MQ=255)
          cTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATAtt                                                   <  1:134675/69‑1 (MQ=255)
           ttGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATAtt                                                   <  1:2384552/68‑1 (MQ=255)
            tGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGTAATATTGAGCGTc                                                              <  1:1106103/56‑1 (MQ=255)
            tGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTc                                                              <  1:1215392/56‑1 (MQ=255)
            tGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTc                                                              <  1:2313110/56‑1 (MQ=255)
                cATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGa                                                       <  1:2788357/59‑1 (MQ=255)
                cATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGa                                                       <  1:2898030/59‑1 (MQ=255)
                cATAATGTTGAATTTCTGAATCTTTAATGTTTGTTACGAAATATTGAGCGTCGACGTGa                                                       <  1:2009462/59‑1 (MQ=255)
                cATAATGTAGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGa                                                       <  1:1051857/59‑1 (MQ=255)
                   aaTGTTGAATTTCTGAATCTTTAATGTTTGTTGCTAAATATTGAGCGTCGACGTGATATTGCTTTCatt                                          >  1:2440647/1‑69 (MQ=255)
                   aaTGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCttt                                              >  1:2278496/1‑65 (MQ=255)
                   aaTGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCatt                                          >  1:1870015/1‑69 (MQ=255)
                    aTGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCatt                                          >  1:271595/1‑68 (MQ=255)
                    aTGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCatt                                          >  1:2003960/1‑68 (MQ=255)
                      gTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCattttt                                       >  1:2580484/1‑69 (MQ=255)
                      gTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCattttt                                       >  1:613254/1‑69 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:3284099/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:2667090/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:170344/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:3276901/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:3354376/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:3414034/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:28309/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATGTTGAGCGTTGACGTGATATTGCTTTCattt                                         <  1:1768463/66‑1 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCattttta                                      >  1:2155580/1‑69 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCattttta                                      >  1:3139826/1‑69 (MQ=255)
                       ttGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCattttta                                      >  1:3194242/1‑69 (MQ=255)
                                tGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAg                              <  1:2913488/68‑1 (MQ=255)
                                tGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAg                              <  1:1501698/68‑1 (MQ=255)
                                tGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAg                              <  1:223301/68‑1 (MQ=255)
                                tGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAg                              <  1:2777195/68‑1 (MQ=255)
                                tGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAg                              <  1:670100/68‑1 (MQ=255)
                                    tCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATc                          <  1:2469817/68‑1 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAtt              >  1:668581/1‑69 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAtt              >  1:1830642/1‑69 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAtt              >  1:1206429/1‑69 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAtt              >  1:1219670/1‑69 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAtt              >  1:3125939/1‑69 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAtt              >  1:2599967/1‑69 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAtt              >  1:1498226/1‑69 (MQ=255)
                                               tgttgCGAAATGTTGAGCGTTGACGTGATATTGCTTTCATTTTTGTTTTTCAGAATCAGTTGCATAAt               >  1:1199488/1‑68 (MQ=255)
                                                      aaaTATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACt       <  1:1106174/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTTTTTTTc                                <  1:443146/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:3230559/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:3154726/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:975617/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:1140012/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:1893281/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:1784503/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:2237371/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:162407/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:2239414/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:2479650/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:2243378/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:175305/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:1723827/41‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:1295768/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:1938745/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:1101500/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:807879/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:188665/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:1785173/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:3326101/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:1716481/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:2528953/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:2543220/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:2980185/69‑1 (MQ=255)
                                                         tatTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGAt    <  1:2969549/69‑1 (MQ=255)
                                                         tatTGAGCGGCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:2383143/41‑1 (MQ=255)
                                                          tttGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:1042003/39‑1 (MQ=255)
                                                          atTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTc                                <  1:1499832/40‑1 (MQ=255)
                                                           ttGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGATTa  >  1:235268/1‑69 (MQ=255)
                                                           ttGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGATTa  >  1:3243685/1‑69 (MQ=255)
                                                           ttGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGATTa  >  1:251643/1‑69 (MQ=255)
                                                           ttGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGATTa  >  1:2651276/1‑69 (MQ=255)
                                                                cGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTg           >  1:2188681/1‑55 (MQ=255)
                                                                cGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTg           >  1:3026661/1‑55 (MQ=255)
                                                                   |                                                            
TTAGTTTCAGCTTGTACATAATGTTGAATTTCTGAATCTTTAATGTTTGTTGCGAAATATTGAGCGTCGACGTGATATTGCTTTCATTTTTATTTTTCAGAATCAGTTGCATAATTTTGAACTGATTA  >  minE/2588844‑2588971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: