| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,591,000 | T→A | 36.0% | V17V (GTT→GTA) | hdeA → | stress response protein acid‑resistance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,591,000 | 0 | T | A | 36.0% | 93.2 / 93.0 | 111 | V17V (GTT→GTA) | hdeA | stress response protein acid‑resistance protein |
| Reads supporting (aligned to +/- strand): ref base T (28/43); new base A (21/19); total (49/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
GAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAC > minE/2590938‑2591054 | gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:244894/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:3192685/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:3226304/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:3144030/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:3076710/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:3244466/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:472894/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:473425/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:3060001/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:2407770/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:2988217/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:2578241/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:136742/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:1275258/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:1102020/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:878390/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:1188474/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:1196130/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:864053/69‑1 (MQ=255)gAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:812233/69‑1 (MQ=255) gATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCaa < 1:1532185/68‑1 (MQ=255) gATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCaa < 1:3054221/68‑1 (MQ=255) atTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCAAGTTGTGAGCAATGCa > 1:1270660/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:1543785/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:1474259/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:506189/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:2174039/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:29474/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:781144/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:1079973/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:951160/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:2098156/1‑69 (MQ=255) ttACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAg > 1:2667646/1‑69 (MQ=255) aCGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCg < 1:966778/69‑1 (MQ=255) aCGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCg < 1:3243709/69‑1 (MQ=255) aCGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCg < 1:1603992/69‑1 (MQ=255) aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTGTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:673046/69‑1 (MQ=255) aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:2060144/69‑1 (MQ=255) aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:1987403/69‑1 (MQ=255) aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:575666/69‑1 (MQ=255) aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:2903176/69‑1 (MQ=255) aaGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCaaa < 1:1026971/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCTCaaaa < 1:3017012/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:629653/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:672743/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:736031/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:766539/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2844800/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2841692/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2823466/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1652952/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1693330/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1447452/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1376486/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1284357/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:1254205/69‑1 (MQ=255) aGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTAGTGAGCAATGCAGCGGATGCACaaaa < 1:2461095/69‑1 (MQ=255) ttAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAagca > 1:284852/1‑69 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAg > 1:2955137/1‑35 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc > 1:1137903/1‑36 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc > 1:1902596/1‑36 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc > 1:712010/1‑36 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc > 1:940293/1‑36 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc > 1:3098746/1‑36 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc > 1:2165765/1‑36 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc > 1:303476/1‑36 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:2216069/1‑68 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:1211019/1‑68 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:1411392/1‑68 (MQ=255) aTTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATaa > 1:289044/1‑68 (MQ=255) ttCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGc < 1:316036/35‑1 (MQ=255) ttCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCg < 1:3204345/44‑1 (MQ=255) cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:495866/69‑1 (MQ=255) cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:952910/69‑1 (MQ=255) cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:3228198/69‑1 (MQ=255) cTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:247092/69‑1 (MQ=255) gCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTc < 1:491841/67‑1 (MQ=255) cttcttCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAc > 1:668183/1‑69 (MQ=255) cttcttCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAc > 1:1042619/1‑69 (MQ=255) cttcttCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAc > 1:1556102/1‑69 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:1617078/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:458694/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:3271568/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2896481/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2057454/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2608252/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:924728/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2060678/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2136088/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:603186/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:843540/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2383094/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:2431618/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGa > 1:920692/1‑47 (MQ=255) cttctGCCAGTAGTGAGCAATGCAGCGGAAGCACAAAAAGCAGCTGa > 1:2140641/1‑47 (MQ=255) ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:723856/1‑63 (MQ=255) ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:1072789/1‑63 (MQ=255) ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:2038352/1‑63 (MQ=255) ttctGCCAGTAGTGAGCAATGCAGCGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:2730912/1‑63 (MQ=255) ttctGCCAGTAGTGAGCAATGCAGAGGATGCACAAAAAGCAGCTGATAACAAAAAACCGGTCa > 1:1389426/1‑63 (MQ=255) | GAGGATATTACGATGAAAAAAGTATTAGGCGTTATTCTTGGTGGTCTGCTTCTTCTGCCAGTTGTGAGCAATGCAGCGGATGCGCAAAAAGCAGCTGATAACAAAAAACCGGTCAAC > minE/2590938‑2591054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |