| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,599,311 | C→T | 58.2% | P279P (CCG→CCA) | yhiR ← | predicted DNA (exogenous) processing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,599,311 | 0 | C | T | 58.2% | 14.8 / 38.9 | 55 | P279P (CCG→CCA) | yhiR | predicted DNA (exogenous) processing protein |
| Reads supporting (aligned to +/- strand): ref base C (17/6); new base T (11/21); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGCGATTGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGCCAC > minE/2599258‑2599374 | aCCGCGATTGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCtt > 1:1633340/1‑69 (MQ=255)aCCGCGATTGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCtt > 1:875294/1‑69 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2292244/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2342791/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2516306/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2093586/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2011386/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2621682/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2755032/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:43919/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:538514/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:1618138/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:936731/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:1482522/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:963697/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:1110278/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:1222081/69‑1 (MQ=255) tGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:1132811/69‑1 (MQ=255) tGTAGCGAGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:98147/69‑1 (MQ=255) gTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCAGGATCCAGCTTACGGTGGc < 1:892393/68‑1 (MQ=255) gTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:1449926/68‑1 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:2252150/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:2219535/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:2201954/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:764197/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:2514524/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:2131912/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:59452/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:1866173/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:3389827/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:300936/1‑69 (MQ=255) gagaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGCGTGCCCgg > 1:2899813/1‑69 (MQ=255) agaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGt < 1:1667293/69‑1 (MQ=255) gaCGTAATAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGt < 1:1810355/68‑1 (MQ=255) aTAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATcc > 1:1395915/1‑40 (MQ=255) aTAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGtgcctgc > 1:1821944/1‑68 (MQ=255) aTAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGtgcctgc > 1:1710121/1‑68 (MQ=255) aTAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGtccctgc > 1:2333928/1‑68 (MQ=255) gCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGtgcctgc > 1:2195047/1‑62 (MQ=255) gCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAAcc > 1:3209276/1‑69 (MQ=255) gCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAAcc > 1:3383328/1‑69 (MQ=255) gCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAAcc > 1:464436/1‑69 (MQ=255) gCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAAcc > 1:791318/1‑69 (MQ=255) gCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAAcc > 1:2358628/1‑69 (MQ=255) gCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTG‑CCGGTGCCTGCCGGAACCa > 1:796209/1‑69 (MQ=255) aTGGCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:3367522/40‑1 (MQ=255) gCTGCAATTACTCTGGCACGATCCAGCTTACGGTGGc < 1:2254700/37‑1 (MQ=255) aTTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGc < 1:3139810/68‑1 (MQ=255) aTTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGc < 1:970186/68‑1 (MQ=255) aTTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGc < 1:820785/68‑1 (MQ=255) ttACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGc < 1:2604652/67‑1 (MQ=255) aCTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGCCAc > 1:2462522/1‑68 (MQ=255) aCTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGCCAc > 1:1576033/1‑68 (MQ=255) aCTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGCCAc > 1:2529265/1‑68 (MQ=255) aCTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGCCAc > 1:288406/1‑68 (MQ=255) | ACCGCGATTGTAGCGCGAGACGTAATAGGTGCCAGCAATGGCTGCAATTACTCCGGCACGATCCAGCTTACGGTGGCGTGCCCGGTGCCTGCCGGAACCAGTTTGCTGTGCAGCCAC > minE/2599258‑2599374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |