| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 223,577 | A→G | 50.7% | G151G (GGA→GGG) | prfH → | predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 223,577 | 0 | A | G | 50.7% | ‑1.6 / 61.3 | 69 | G151G (GGA→GGG) | prfH | predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base A (10/24); new base G (10/25); total (20/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTA > minE/223522‑223639 | aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGa < 1:564295/69‑1 (MQ=255)aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGa < 1:242571/69‑1 (MQ=255)aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGa < 1:3200656/69‑1 (MQ=255)aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGa < 1:1970346/69‑1 (MQ=255)aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGa < 1:1511878/69‑1 (MQ=255)aGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGa < 1:1470666/69‑1 (MQ=255) aCCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCg < 1:2592441/69‑1 (MQ=255) aCCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCg < 1:3427487/69‑1 (MQ=255) aCCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCg < 1:1264747/69‑1 (MQ=255) aCCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCg < 1:798941/69‑1 (MQ=255) gcgACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa < 1:803609/69‑1 (MQ=255) gcgACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa < 1:872464/69‑1 (MQ=255) gcgACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa < 1:1672686/69‑1 (MQ=255) gcgACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa < 1:185654/69‑1 (MQ=255) gcgACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa < 1:33629/69‑1 (MQ=255) aCGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa < 1:670004/66‑1 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:171233/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:2093337/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:2375438/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:413148/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:719730/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:2561248/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:3362934/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc > 1:1467085/1‑67 (MQ=255) tACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATc > 1:2104993/1‑66 (MQ=255) gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcag < 1:3315435/68‑1 (MQ=255) gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcag < 1:2296215/68‑1 (MQ=255) gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcag < 1:2899799/68‑1 (MQ=255) gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcag < 1:2173538/68‑1 (MQ=255) gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcag < 1:2142724/68‑1 (MQ=255) aTGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCata < 1:2698317/69‑1 (MQ=255) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcatcaaa < 1:379965/69‑3 (MQ=38) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:554427/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:3256902/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:2276868/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:2681157/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:3339109/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:568183/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:1021494/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:2446904/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:222986/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:2068552/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:1219769/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:1330902/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:754354/69‑3 (MQ=39) aTGGCCTTTATCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcagcaaa < 1:145389/69‑3 (MQ=39) gCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa > 1:2950743/1‑47 (MQ=255) tttATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGtcagca < 1:3064752/61‑1 (MQ=255) tttATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGtcagca < 1:3068645/61‑1 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2030019/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:1125592/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:1611122/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2026130/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2053042/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2656521/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:1979153/1‑68 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:808275/1‑68 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:3263737/1‑68 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:1323228/1‑68 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCCAATAATTa < 1:1038117/68‑1 (MQ=39) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:515245/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:2636626/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:2477807/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1430513/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1359147/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1293488/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:878786/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGAATAAACGCCATCCGGCAGCTAATCAAATAATTa < 1:3414537/68‑1 (MQ=255) aaGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1603131/67‑1 (MQ=255) | AGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTA > minE/223522‑223639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |