| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,601,906 | T→C | 100% | S519S (TCT→TCC) | prlC → | oligopeptidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,601,906 | 0 | T | C | 81.9% | 11.5 / ‑2.3 | 11 | S519S (TCT→TCC) | prlC | oligopeptidase A |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (0/9); total (2/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAACTGCCGAGTCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCTGGTCACTATGAAACCGGCGAACCGCTGCCGAAAGAGTTGCTGGATAAAATGCTGGCGGCGA > minE/2601844‑2601967 | gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:1567383/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:1641444/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:2237398/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:2437462/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:254369/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:2657755/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:2928549/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:3107667/69‑1 (MQ=255)gAACTGCCGAGCCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCCGGTCAc < 1:3165792/69‑1 (MQ=255) ggCGCTGGCGTTTATCTCTGGTCACTATGAAACCGGCGAACCGCTGCCGAAAGAGTTGCTGGATAAAAt > 1:2016931/1‑69 (MQ=255) tATCTCTGGTCACTATGAAACCGGCGAACCGCTGCCGAAAGAGTTGCTGGATAAAATGCTGGCGGCga > 1:2556058/1‑68 (MQ=255) | GAACTGCCGAGTCAGTTTATGGAAAACTGGTGCTGGGAGCCGGAGGCGCTGGCGTTTATCTCTGGTCACTATGAAACCGGCGAACCGCTGCCGAAAGAGTTGCTGGATAAAATGCTGGCGGCGA > minE/2601844‑2601967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |