Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,604,020 C→T 37.4% V217V (GTG→GTA)  yhiP ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,604,0200CT37.4% 86.8 / 86.3 136V217V (GTG→GTA) yhiPpredicted transporter
Reads supporting (aligned to +/- strand):  ref base C (70/15);  new base T (23/28);  total (93/43)
Fisher's exact test for biased strand distribution p-value = 9.40e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

GATTGGCGACTTCTACGTTGTGCATCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCAT  >  minE/2603953‑2604085
                                                                   |                                                                 
gATTGGCGACTTCTACGTTGTGCATCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAaca                                                                  <  1:1456739/69‑1 (MQ=255)
gATTGGCGACTTCTACGTTGTGCATCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAaca                                                                  <  1:2970556/69‑1 (MQ=255)
                 ttGTGCATCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTg                                                 <  1:208238/69‑1 (MQ=255)
                 ttGTGCATCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTg                                                 <  1:1390075/69‑1 (MQ=255)
                 ttGTGCATCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTg                                                 <  1:2934312/69‑1 (MQ=255)
                        tCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCa                                          >  1:2855968/1‑69 (MQ=255)
                        tCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCa                                          >  1:678679/1‑69 (MQ=255)
                        tCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCa                                          >  1:1703450/1‑69 (MQ=255)
                        tCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCa                                          >  1:2405246/1‑69 (MQ=255)
                        tCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCa                                          >  1:16208/1‑69 (MQ=255)
                        tCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCa                                          >  1:2639914/1‑69 (MQ=255)
                                 cgcATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGa                                 >  1:3382823/1‑69 (MQ=255)
                                 cgcATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGa                                 >  1:3227864/1‑69 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCTGCCGGaa                                <  1:27614/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2635315/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2462364/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2746748/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:3050815/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2770940/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2240414/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2782675/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:3175220/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2815567/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:3230180/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2027669/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:2000428/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:811273/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:3390598/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:426705/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:1832805/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:490707/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:1804689/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:1779084/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:1452294/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:1311349/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:1765088/69‑1 (MQ=255)
                                  gcAGACGAAGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGaa                                <  1:1673443/69‑1 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:641266/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:523391/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:270056/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:3016312/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:1923934/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:2397765/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:2315329/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:2206949/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTacaaca                                                         >  1:2130812/1‑39 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTc                                           >  1:308480/1‑53 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTc                                           >  1:1200073/1‑53 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCgg                           >  1:1650696/1‑69 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCgg                           >  1:585701/1‑69 (MQ=255)
                                       cGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCgg                           >  1:3096504/1‑69 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGACGCTCATCGGCCGGAAGTCggg                          >  1:2494773/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAg                                              >  1:3332698/1‑47 (MQ=38)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2898475/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2894385/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:512508/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:669173/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:74710/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:77133/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:884031/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2655294/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2031786/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2031917/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:157579/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2329265/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:1363849/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2527930/1‑67 (MQ=255)
                                          aGATCATCACCACGCTGCCAAGTAATACGTACAACAGTTTACTGAAGCTCATCGGCCGGAAGTCggg                          >  1:2560527/1‑67 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:592337/69‑1 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:588055/69‑1 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:1091199/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:525344/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:1839790/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:364648/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:343032/69‑1 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:279910/69‑1 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:3407919/69‑1 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:1981055/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:1088840/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:3098701/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:2652171/69‑1 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:2904647/69‑1 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:2230142/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:2062905/1‑69 (MQ=255)
                                             tcatcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     <  1:2058425/69‑1 (MQ=255)
                                             tcaacaCCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAg                     >  1:3158687/1‑69 (MQ=255)
                                              catcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCt                                                  >  1:1866029/1‑39 (MQ=255)
                                              catcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCt                                                  >  1:1562317/1‑39 (MQ=255)
                                              catcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCt                                                  >  1:3292250/1‑39 (MQ=255)
                                              catcaCCACGCTGCCAAGTAACACGTACAACAGTTTGCt                                                  >  1:1823670/1‑39 (MQ=255)
                                                  accacGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAAcc                 >  1:3089130/1‑68 (MQ=255)
                                                  accacGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAAcc                 >  1:1132375/1‑68 (MQ=255)
                                                             aaGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCAc     <  1:1194971/69‑1 (MQ=255)
                                                                tAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCa   >  1:2133343/1‑68 (MQ=255)
                                                                tAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCAt  >  1:3246523/1‑69 (MQ=255)
                                                                 aaCACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATg             >  1:1605389/1‑57 (MQ=255)
                                                                 aaCACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCAt  >  1:1612754/1‑68 (MQ=255)
                                                                 aaCACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCAt  >  1:490812/1‑68 (MQ=255)
                                                                 aaCACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCAt  >  1:2429779/1‑68 (MQ=255)
                                                                 aaCACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCAt  >  1:1184118/1‑68 (MQ=255)
                                                                   |                                                                 
GATTGGCGACTTCTACGTTGTGCATCAGCCATGCGCATACGAAGATCATCACCACGCTGCCAAGTAACACGTACAACAGTTTGCTGAAGCTCATTGGCCGGAAGTCGGGTTCAGAACCAATGTCTTTCACCAT  >  minE/2603953‑2604085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑