| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,665 | A→C | 33.6% | V443V (GTA→GTC) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,665 | 0 | A | C | 33.6% | 104.7 / 108.7 | 134 | V443V (GTA→GTC) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base A (42/47); new base C (11/34); total (53/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01 | |||||||||||
Only 100 of 139 total aligned reads displayed.
AATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTA > minE/1606‑1732 | aaTATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:1834485/69‑1 (MQ=255)aaTATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:2851375/69‑1 (MQ=255)aaTATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:2773508/69‑1 (MQ=255)aaTATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:949031/69‑1 (MQ=255)aaTATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:2617138/69‑1 (MQ=255)aaTATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:20619/69‑1 (MQ=255) atatCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:1307525/68‑1 (MQ=255) tCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgatgat > 1:1235010/1‑68 (MQ=255) tCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgatgat > 1:2122549/1‑68 (MQ=255) tCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgatgat > 1:1935487/1‑68 (MQ=255) tCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:2847486/65‑1 (MQ=255) aaCATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCg > 1:1496799/1‑69 (MQ=255) gTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACt > 1:1047345/1‑69 (MQ=255) gTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACt > 1:383642/1‑69 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCGt > 1:567089/1‑68 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCGTg > 1:2703972/1‑69 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCGTg > 1:2990284/1‑69 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCGTg > 1:572579/1‑69 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCGTg > 1:855227/1‑69 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAACGATGATGCGACCACTGGCGTg > 1:1166596/1‑69 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTCAATAAAGATGATGCGACCACTGGCGTg > 1:2229966/1‑69 (MQ=255) aTTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTg < 1:3049758/69‑1 (MQ=255) tGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTgcg > 1:2433766/1‑69 (MQ=255) tGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTgc > 1:1807028/1‑68 (MQ=255) gCTGAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:1586014/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:3296027/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:1255095/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:2660480/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:2180813/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:1988892/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:2722066/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:3364516/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:103353/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:65092/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat > 1:2854018/1‑48 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACga > 1:1814442/1‑47 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTgcgc > 1:1455621/1‑69 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTgcgc > 1:1940917/1‑69 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTgcgc > 1:281943/1‑69 (MQ=255) gCTCAGGGATCTTCTGAACGCTCAATCTCAGTCGTGGAAAATAACgat > 1:2420158/1‑48 (MQ=255) ggATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTAc > 1:2008719/1‑68 (MQ=255) ggATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTAc > 1:1823622/1‑68 (MQ=255) ggATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACt > 1:449772/1‑69 (MQ=255) aTCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACgat < 1:1224980/40‑1 (MQ=255) cttctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtc > 1:2455374/1‑66 (MQ=255) cttctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtc > 1:2264882/1‑66 (MQ=255) gAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 1:162776/65‑1 (MQ=255) gAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 1:3376067/65‑1 (MQ=255) gAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 1:633818/65‑1 (MQ=255) gAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 1:546164/65‑1 (MQ=255) gAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 1:1908995/65‑1 (MQ=255) gAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 1:459952/65‑1 (MQ=255) gAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca < 1:925908/65‑1 (MQ=255) tCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTc < 1:3393605/69‑1 (MQ=255) tCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTc < 1:115593/69‑1 (MQ=255) tCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTc < 1:3355718/69‑1 (MQ=255) cAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca > 1:3137445/1‑58 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:377914/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:3275058/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:921385/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:3194633/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:3278714/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:824169/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:512137/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:69195/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:952489/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:2783189/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1680813/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1765801/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1517803/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1965131/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:2054166/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1324065/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1256542/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:2224376/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:2366380/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:2347995/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1171658/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:2587970/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:2799519/67‑1 (MQ=255) gTGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:268125/67‑1 (MQ=255) gTGGTCAATAAAGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:156797/67‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:725030/68‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:3147618/68‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:1780050/68‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:555975/68‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:1783972/68‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:727680/68‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:3423095/68‑1 (MQ=255) gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCa < 1:923257/68‑1 (MQ=255) tGGTCAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc < 1:1532401/66‑1 (MQ=255) gTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGtt < 1:1078727/69‑1 (MQ=255) gTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGtt < 1:1834394/69‑1 (MQ=255) gTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGtt < 1:1948063/69‑1 (MQ=255) gTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGtt < 1:2205636/69‑1 (MQ=255) gTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGtt < 1:2547604/69‑1 (MQ=255) gTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGtt < 1:2634018/69‑1 (MQ=255) aaaTAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTa < 1:139926/68‑1 (MQ=255) aaaTAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTa < 1:207223/68‑1 (MQ=255) aaaTAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTa < 1:3378461/68‑1 (MQ=255) | AATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTA > minE/1606‑1732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |