| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 28,911 | T→C | 33.3% | I139I (ATT→ATC) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 28,911 | 0 | T | C | 33.3% | 56.8 / 40.0 | 75 | I139I (ATT→ATC) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (33/17); new base C (21/4); total (54/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01 | |||||||||||
TGCCACTGCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGACG > minE/28845‑28978 | tGCCACTAACGATACGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTg < 1:2271783/68‑1 (MQ=255) tGCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGa > 1:2289096/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGCCGTAGCGATGAAGAAAATTGGTCTGGaa > 1:1186057/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2957700/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2274541/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2535721/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2625017/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2781537/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2834490/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2935317/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2741196/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:3021128/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:316107/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:3190657/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:3274641/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:445573/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:657651/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:993821/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:1855122/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:1124829/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:1363758/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:1536973/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:1720787/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCAGGaa > 1:1481257/1‑69 (MQ=255) gCCGATGCGATTGATAAAGCAGAAGACAGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:1082443/1‑69 (MQ=255) gCCGATACGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGaa > 1:2235/1‑69 (MQ=255) tGCGATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACtgc < 1:3105160/69‑1 (MQ=255) tGCGATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACtgc < 1:3098739/69‑1 (MQ=255) tGCGATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACtgc < 1:3052764/69‑1 (MQ=255) tGCGATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACtgc < 1:3023660/69‑1 (MQ=255) gaagaCCGCCGTCGTTTCGCCGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGca > 1:1891921/1‑69 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGATGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGcacac > 1:2452215/1‑69 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGcacac > 1:2707994/1‑69 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGcacac > 1:538553/1‑69 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGcacac > 1:2899471/1‑69 (MQ=255) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:3299193/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1215641/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1262207/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:31166/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1984240/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:3047085/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1163105/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1849401/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1464513/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1802774/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:2880289/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:2854361/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:770544/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:2831336/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1624198/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:2775678/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:2720360/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:1656523/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:2544170/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGaa > 1:2482673/1‑40 (MQ=38) ccgccgTTTCGACGTAGCGATGAAGAAAATCGGTCTGGa > 1:3117627/1‑39 (MQ=37) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:670679/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:835345/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:404553/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:950284/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:210307/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:1203445/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:2209339/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:2908339/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:1737967/49‑1 (MQ=255) gtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:2325127/49‑1 (MQ=255) ctttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg < 1:3324306/48‑1 (MQ=255) gCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCg > 1:348272/1‑69 (MQ=255) gCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCg > 1:2839256/1‑69 (MQ=255) gCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCg > 1:826836/1‑69 (MQ=255) aTGAAGAAAATTGGGCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGtt < 1:3339003/69‑1 (MQ=255) aaaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCt < 1:1999153/69‑1 (MQ=255) aaaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCt < 1:657610/69‑1 (MQ=255) aaaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCt < 1:1962675/69‑1 (MQ=255) aaaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCt < 1:1035517/69‑1 (MQ=255) ttGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGACg < 1:1954962/69‑1 (MQ=255) | TGCCACTGCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGACG > minE/28845‑28978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |