| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 223,627 | T→A | 29.4% | intergenic (+47/+10) | prfH → / ← pepD | predicted peptide chain release factor/aminoacyl‑histidine dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 223,627 | 0 | T | A | 29.4% | 85.1 / 42.1 | 68 | intergenic (+47/+10) | prfH/pepD | predicted peptide chain release factor/aminoacyl‑histidine dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (14/34); new base A (10/10); total (24/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATG > minE/223560‑223687 | aTGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCata < 1:2698317/69‑1 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2656521/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:1125592/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2053042/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2030019/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:2026130/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat > 1:1611122/1‑69 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:808275/1‑68 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:1323228/1‑68 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:3263737/1‑68 (MQ=255) aTCGAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa > 1:1979153/1‑68 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCCAATAATTa < 1:1038117/68‑1 (MQ=39) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:878786/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:2636626/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:2477807/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1430513/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1359147/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:515245/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1293488/68‑1 (MQ=255) gAAGGGTAATGAAGGATGAAACTGCCGGAAGGCGAATAAACGCCATCCGGCAGCTAATCAAATAATTa < 1:3414537/68‑1 (MQ=255) aaGGGTAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTa < 1:1603131/67‑1 (MQ=255) tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc > 1:368901/1‑63 (MQ=255) tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc > 1:2990838/1‑63 (MQ=255) tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc > 1:3231918/1‑63 (MQ=255) tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc > 1:825590/1‑63 (MQ=255) tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc > 1:345110/1‑63 (MQ=255) aaGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGaa > 1:2884471/1‑68 (MQ=255) aTGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCata < 1:1025235/42‑1 (MQ=255) aCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcag < 1:3307115/69‑1 (MQ=255) aCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcag < 1:2337587/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc < 1:1932011/46‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTAc < 1:832121/46‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:992779/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:851320/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:2740682/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:2796/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:3241507/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:2568015/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:233309/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagcag < 1:2207856/69‑1 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagc > 1:260282/1‑67 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagc > 1:1017147/1‑67 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagc > 1:562458/1‑67 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagc > 1:428721/1‑67 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagc > 1:1648284/1‑67 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTcagc > 1:1945381/1‑67 (MQ=255) gCCGGAAGGCGATTAAACGCCATCCGGCAGAATATCAAATAATTACTTCGCCGGAATTTCTTTcagc > 1:876988/1‑67 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:2715266/69‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:955304/69‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:953898/69‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:1023160/69‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:2011449/69‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:2258195/69‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:3075061/69‑1 (MQ=255) gAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtca < 1:3104816/69‑1 (MQ=255) tAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAgt > 1:1008141/1‑69 (MQ=255) ccGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAAt < 1:2002442/69‑1 (MQ=255) ccGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAAt < 1:3004847/69‑1 (MQ=255) ccGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAAt < 1:1492095/69‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:1336155/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:3110107/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:1287668/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:480687/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:1011135/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:2940267/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:217442/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:962682/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGt < 1:206034/65‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATg < 1:1285743/69‑1 (MQ=255) cGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATg < 1:3180317/69‑1 (MQ=255) | ATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCATATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATG > minE/223560‑223687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |