| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,606,682 | G→A | 32.8% | R403R (CGC→CGT) | pitA ← | phosphate transporter, low‑affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,606,682 | 0 | G | A | 32.8% | 75.3 / 44.0 | 88 | R403R (CGC→CGT) | pitA | phosphate transporter, low‑affinity |
| Reads supporting (aligned to +/- strand): ref base G (35/24); new base A (9/20); total (44/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
GACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATA > minE/2606617‑2606750 | gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCAGCGATAGTCGTTGCCACACGgc > 1:1863842/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:1128436/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:869297/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:2721756/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:3413255/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:2894367/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:202279/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:3313561/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGgc > 1:595347/1‑67 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGacgc < 1:1061554/69‑1 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGacgc < 1:218400/69‑1 (MQ=255)gACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGacgc < 1:3232928/69‑1 (MQ=255) aCATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGacgc < 1:147560/68‑1 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:655438/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:1375470/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:1215440/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:1488954/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:435545/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:672947/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:3061293/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:2449846/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:3369615/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcca > 1:1845/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCgccagcc > 1:2000575/1‑68 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCaccagcca > 1:1826995/1‑69 (MQ=255) cccTGAGCGTAGGTCATGCCTTTCTTACCGATTTTATCACCGATAGTCGTTGCCACACGGCgccagcca > 1:783486/1‑69 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:2247169/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:2747419/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:252071/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:2783101/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:710738/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:68469/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:3177301/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:2240971/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:1353177/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:367961/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:3347648/69‑1 (MQ=255) cGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:351320/69‑1 (MQ=255) gTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATCGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:1632224/68‑1 (MQ=255) gTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGt < 1:3049350/69‑1 (MQ=255) gTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGt < 1:1045971/69‑1 (MQ=255) gTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:1785235/68‑1 (MQ=255) tAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:3364050/67‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:573086/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:3365828/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:3112087/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:778779/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:2751482/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:1152551/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:249292/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:2435580/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:240038/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:1155572/69‑1 (MQ=255) cATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGAt < 1:1193737/69‑1 (MQ=255) ccTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATAcc > 1:1730951/1‑68 (MQ=255) ccTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCt > 1:74034/1‑69 (MQ=255) ccTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCt > 1:3309725/1‑69 (MQ=255) ccTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCt > 1:1369189/1‑69 (MQ=255) ccTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCt > 1:844388/1‑69 (MQ=255) ccTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCt > 1:2637564/1‑69 (MQ=255) ccTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCt > 1:870721/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTa > 1:2203228/1‑51 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:1189106/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:2723630/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:559885/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:552868/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:2147612/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:3286986/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:3067119/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:3068332/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:2336847/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAgc > 1:2268511/1‑69 (MQ=255) ccGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAg > 1:2374396/1‑68 (MQ=255) ttCTCACCGATAGTCGTTGCCACACGACGCCAGCCAATCATCg < 1:1835090/43‑1 (MQ=255) gTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATc < 1:2740229/69‑1 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:3189977/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:2768888/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:2865643/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:1167832/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:143045/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:2139703/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:391197/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:3425538/1‑69 (MQ=255) tGCCACACGACGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCacac > 1:369295/1‑69 (MQ=255) cacGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTg < 1:1547312/69‑1 (MQ=255) cacGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTg < 1:2020784/69‑1 (MQ=255) cacGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTg < 1:1789249/69‑1 (MQ=255) cacGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTg < 1:1163531/69‑1 (MQ=255) cacGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTg < 1:1490821/69‑1 (MQ=255) cGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCa < 1:3148658/69‑1 (MQ=255) cGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCa < 1:513230/69‑1 (MQ=255) cGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCa < 1:451307/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:1349066/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:1715102/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:584652/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:765339/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:419294/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:78117/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:330941/69‑1 (MQ=255) gcgcCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATa < 1:3362348/69‑1 (MQ=255) | GACATCCCCTGAGCGTAGGTCATGCCTTTCTTACCGATTTTCTCACCGATAGTCGTTGCCACACGGCGCCAGCCAATCATCGTACCGATACCTAACGCCAGCGCGACCGCCATGATGATCCACACCGGTGCATA > minE/2606617‑2606750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |