Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,606,931 A→G 38.8% G320G (GGT→GGC pitA ← phosphate transporter, low‑affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,606,9310AG38.8% 48.7 / 68.4 102G320G (GGT→GGCpitAphosphate transporter, low‑affinity
Reads supporting (aligned to +/- strand):  ref base A (33/29);  new base G (3/36);  total (36/66)
Fisher's exact test for biased strand distribution p-value = 1.62e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 131 total aligned reads displayed.

ATGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGGTTGCGTT  >  minE/2606863‑2606997
                                                                    |                                                                  
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    <  1:2222641/69‑1 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:3195348/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:2043421/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:2139613/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:2207593/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:3110471/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:3422647/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:1715945/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:3424526/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:357743/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:1434189/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:496070/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:1362481/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:1355695/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:2306773/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:2917205/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:694034/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    <  1:2563105/69‑1 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:1037388/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:1066294/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:2832683/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:1256716/1‑69 (MQ=255)
aTGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATa                                                                    >  1:2827362/1‑69 (MQ=255)
  gcggcgCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATAcc                                                                  <  1:1892097/69‑1 (MQ=255)
  gcggcgCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATAcc                                                                  <  1:1014233/69‑1 (MQ=255)
       gcATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCa                                                             >  1:1983495/1‑69 (MQ=255)
       gcATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCa                                                             >  1:3175924/1‑69 (MQ=255)
       gcATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCa                                                             >  1:1192758/1‑69 (MQ=255)
       gcATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCa                                                             >  1:1245484/1‑69 (MQ=255)
          tCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGc                                                          >  1:1722293/1‑69 (MQ=255)
          tCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGc                                                          >  1:2004266/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTTGTCAACATACCTTTCAGGCgg                                                        >  1:1737344/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:1772442/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:2205701/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:2128249/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:2065855/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:2631011/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:1824104/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:1098444/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:2473276/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:1449360/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:563069/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:1365214/1‑69 (MQ=255)
            tGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCgg                                                        >  1:1254381/1‑69 (MQ=255)
             ggCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCgg                                                        >  1:2635176/1‑68 (MQ=255)
             ggCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCgg                                                        >  1:1823971/1‑68 (MQ=255)
             ggCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCgg                                                        >  1:1134932/1‑68 (MQ=255)
                    ttGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATTCCTTTCAGGCGGTTGAgcg                                                 <  1:2248386/68‑1 (MQ=255)
                    ttGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAgcg                                                 <  1:1001134/68‑1 (MQ=255)
                    ttGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAgcg                                                 <  1:492337/68‑1 (MQ=255)
                        tCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGtt                                             <  1:3259624/68‑1 (MQ=255)
                        tCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGtt                                             <  1:714958/68‑1 (MQ=255)
                        tCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGtt                                             <  1:183933/68‑1 (MQ=255)
                        tCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGtt                                             <  1:1263096/68‑1 (MQ=255)
                            gCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:3029564/69‑1 (MQ=255)
                            gCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:814692/69‑1 (MQ=255)
                            gCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:605747/69‑1 (MQ=255)
                                 aGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTc                                   <  1:2490212/69‑1 (MQ=255)
                                     tGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACg                               <  1:2108326/69‑1 (MQ=255)
                                     tGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACg                               <  1:2998333/69‑1 (MQ=255)
                                     tGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACg                               <  1:1181000/69‑1 (MQ=255)
                                     tGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACg                               <  1:1102146/69‑1 (MQ=255)
                                     tGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACg                               <  1:1929465/69‑1 (MQ=255)
                                     tGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACg                               <  1:3419062/69‑1 (MQ=255)
                                     tGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACg                               <  1:2045124/69‑1 (MQ=255)
                                         gTAGCTTTCCAGATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:921156/56‑1 (MQ=38)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:878388/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:3280058/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:3354995/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:2080789/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:1961599/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:1431009/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:3112437/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:1426692/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:1372899/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:3024263/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:68281/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:2547475/56‑1 (MQ=255)
                                         gTAGCTTTCCACATCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGTTAATgg                                        <  1:2752202/56‑1 (MQ=255)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:1317902/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:1219852/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:1037186/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:2564204/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:3193643/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:2625363/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:2425477/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:2360335/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:2316039/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:2306974/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:3061858/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTGAGCGCGtt                                             <  1:2210626/40‑1 (MQ=38)
                                                    caTCGGTAGTCAACATGCCTTTCAGGCGGTTAGCGCGtt                                             <  1:1015253/39‑1 (MQ=21)
                                                              cAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttg      >  1:2221651/1‑69 (MQ=255)
                                                              cAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttg      >  1:750518/1‑69 (MQ=255)
                                                              cAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttg      >  1:1001670/1‑69 (MQ=255)
                                                                  aTACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttgcgtt  >  1:481325/1‑69 (MQ=255)
                                                                  aTACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttgcgtt  >  1:1619467/1‑69 (MQ=255)
                                                                  aTACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttgcgtt  >  1:1782719/1‑69 (MQ=255)
                                                                  aTACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttgcgtt  >  1:1974628/1‑69 (MQ=255)
                                                                  aTACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGgttgcgt   >  1:1631045/1‑68 (MQ=255)
                                                                    |                                                                  
ATGCGGCGCATCTGGCTACGTTGATCAAGCGACAGCTTGTCGTAGCTTTCCACATCGGTGGTCAACATACCTTTCAGGCGGTTGAGCGCGTTAATGGTATTCGACGGATGGCAGTGGAACTCCGCAGGTTGCGTT  >  minE/2606863‑2606997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: