| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,609,041 | A→C | 51.3% | S125A (TCC→GCC) | nikE ← | nickel transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,609,041 | 0 | A | C | 51.3% | 12.2 / 89.0 | 82 | S125A (TCC→GCC) | nikE | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (19/21); new base C (13/29); total (32/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
AACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCG > minE/2608982‑2609103 | aaCGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCa > 1:903532/1‑68 (MQ=255)aaCGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCa > 1:364695/1‑68 (MQ=255) aCGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGt < 1:2948544/69‑1 (MQ=255) aCGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGt < 1:1987982/69‑1 (MQ=255) aCGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGt < 1:1510551/69‑1 (MQ=255) aCGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGt < 1:3338458/69‑1 (MQ=255) aCGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGt < 1:2794233/69‑1 (MQ=255) cGAGATCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:2519016/69‑1 (MQ=255) cGAGATCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:54582/69‑1 (MQ=255) cGAGATCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:2195212/69‑1 (MQ=255) cGAGATCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:2891543/69‑1 (MQ=255) cGAGATCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:879539/69‑1 (MQ=255) cGAGATCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:2109396/69‑1 (MQ=255) gagaTCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:1126324/68‑1 (MQ=255) gagaTCGACCGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:1631808/68‑1 (MQ=255) ccGCCTTCAGCATTTCACTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGCGATAGCAGGt < 1:1069421/60‑1 (MQ=255) cTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGt < 1:2402276/46‑1 (MQ=255) cAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCa > 1:1526053/1‑67 (MQ=255) cAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCa > 1:920148/1‑67 (MQ=255) cAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCa > 1:40801/1‑67 (MQ=255) cAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCa > 1:1681964/1‑67 (MQ=255) aGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGt < 1:2099985/42‑1 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAgg < 1:2538344/66‑1 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAgg < 1:3306171/66‑1 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAgg < 1:1582782/66‑1 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAgg < 1:1787739/66‑1 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAgg < 1:16313/66‑1 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAgg < 1:1749744/66‑1 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGAtc > 1:2761785/1‑69 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGAtc > 1:2635612/1‑69 (MQ=255) tCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGAt > 1:155804/1‑68 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGTCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:1817470/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:2791509/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:183580/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:2692281/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:1278998/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:3309871/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:2631107/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:1405535/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:933585/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:1507365/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:910291/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:452370/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:464259/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:514779/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:579620/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:708175/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:828141/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGAGCTCGCGCACGGTTTTg < 1:2530828/69‑1 (MQ=255) aGTTGTTCGGCTTTTTTCAGCGACAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:3070536/69‑1 (MQ=255) aGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGtttt > 1:356861/1‑68 (MQ=255) aGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg > 1:1639663/1‑69 (MQ=255) aGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg > 1:1880358/1‑69 (MQ=255) gttgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTg < 1:2904514/68‑1 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGc > 1:2941500/1‑45 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:949112/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:585741/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:254982/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:1045604/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:1927292/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:2617168/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:2573225/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:256523/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:1021708/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:1952121/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:237696/1‑46 (MQ=255) tgttCGGCTTTTTTCAGCGATAGCAGGTGGCGCATCGGTTCACGCa > 1:2013588/1‑46 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCa > 1:2175930/1‑57 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCTCGGt < 1:1538559/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGt < 1:1722485/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGt < 1:1687247/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGt < 1:2938939/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGt < 1:1575904/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGt < 1:2398687/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGt < 1:2870271/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGt < 1:1056239/61‑1 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGcgcg > 1:2344185/1‑69 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGcgcg > 1:714885/1‑69 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGcgcg > 1:2348755/1‑69 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGcgcg > 1:2899730/1‑69 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGcgcg > 1:3243252/1‑69 (MQ=255) gttCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGcgcg > 1:3237035/1‑69 (MQ=255) | AACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCG > minE/2608982‑2609103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |