| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,609,188 | G→A | 43.1% | L76L (CTG→TTG) | nikE ← | nickel transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,609,188 | 0 | G | A | 43.1% | 11.3 / 29.3 | 44 | L76L (CTG→TTG) | nikE | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (17/8); new base A (13/6); total (30/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTCGCCACGCCAGCTAATATTCCCCTGCGCGGGCGATTCTAAACCCACCAGCAACCGC > minE/2609126‑2609249 | cTGAAATACCATTTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTc > 1:645122/1‑69 (MQ=255)cTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTc > 1:419966/1‑69 (MQ=255)cTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTc > 1:349043/1‑69 (MQ=255)cTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTc > 1:3419831/1‑69 (MQ=255)cTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTc > 1:3167416/1‑69 (MQ=255)cTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTc > 1:1777011/1‑69 (MQ=255)cTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTc > 1:2074092/1‑69 (MQ=255) aTACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccac > 1:2028208/1‑69 (MQ=255) aCCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgc < 1:571817/64‑1 (MQ=255) aCCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgc < 1:619794/64‑1 (MQ=255) aCCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgc < 1:3004084/64‑1 (MQ=255) ccATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccacgcc > 1:2408594/1‑69 (MQ=255) ccATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccacgcc < 1:349266/69‑1 (MQ=255) ccATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccacgcc < 1:695482/69‑1 (MQ=255) ccATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccacgcc > 1:2514322/1‑69 (MQ=255) ccATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccacgcc > 1:250724/1‑69 (MQ=255) ccATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccacgcc > 1:2305269/1‑69 (MQ=255) cTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgc < 1:323233/59‑1 (MQ=255) tGAATATCGCGGCGGAACGCTTTTCGCTGGGCGCGATTGAGTTTCGCCAGCGGTTCGCCACGCCAGCt > 1:1468528/1‑68 (MQ=255) tGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTCGCCACGCCAGCt > 1:876608/1‑68 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:114368/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:559092/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:430568/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:1244141/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:1389446/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:1506691/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:1555281/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:1682117/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:1702888/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:1907399/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:648420/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:2043658/1‑48 (MQ=255) tatCACGGCGGAACGCTTTACGTTGCGCGCGATTGAGCTTCGCCAACg > 1:730092/1‑48 (MQ=255) gcggAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTcgccacgc > 1:32005/1‑52 (MQ=255) gcggAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTCGCCACGCCAGCTAATATTCCCCt > 1:2070171/1‑68 (MQ=255) tGAGTTTCGCCAGCGGTTCGCCACGCCAGCTAATATTCCCCTGCGCGGGCGATTCTAAAcc < 1:383514/61‑1 (MQ=255) gAGTTTCGCCAGCGGGTCGCCACGCCAGCTAATATTCCCCTGCGCGGGCGATTCTAAAcc < 1:1218795/60‑1 (MQ=255) tCGCCAGCGGTTCGCCACGCCAGCTAATATTCCCCTGCGCGGGCGATTCTAAACCCACCAGCAACcgc > 1:2597910/1‑68 (MQ=255) gCCAACGGTTCGCCGCGCCAGCTAATATTTCCCTGTTAGGGCGACTCTAAccct < 1:2320981/54‑2 (MQ=255) gCCAACGGTTCGCCGCGCCAGCTAATATTTCCCTGTGAGGGCGACTCTAAccct < 1:3417437/54‑2 (MQ=255) gCCAACGGTTCGCCGCGCCAGCTAATATTTCCCTGTGAGGGCGACTCTAAccct < 1:3279106/54‑2 (MQ=255) gCCAACGGTTCGCCGCGCCAGCTAATATTTCCCTGTGAGGGCGACTCTAAccct < 1:299/54‑2 (MQ=255) gCCAACGGTTCGCCGCGCCAGCTAATATTTCCCTGTGAGGGCGACTCTAAccct < 1:765125/54‑2 (MQ=255) gCCAACGGTTCGCCGCGCCAGCTAATATTTCCCTGTGAGGGCGACTCTAAccct < 1:2124894/54‑2 (MQ=255) | CTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGGCGCGATTGAGTTTCGCCAGCGGTTCGCCACGCCAGCTAATATTCCCCTGCGCGGGCGATTCTAAACCCACCAGCAACCGC > minE/2609126‑2609249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |