| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,609,767 | C→G | 35.7% | L136L (CTG→CTC) | nikD ← | nickel transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,609,767 | 0 | C | G | 35.7% | 45.9 / 45.0 | 56 | L136L (CTG→CTC) | nikD | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (16/20); new base G (11/9); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGC > minE/2609707‑2609829 | caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:468615/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:225641/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg > 1:94267/1‑69 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:1999453/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:2304220/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg > 1:2339526/1‑69 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg > 1:2355009/1‑69 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg > 1:2409328/1‑69 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:782109/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg > 1:781389/1‑69 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:3429846/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:127422/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:1264568/69‑1 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg > 1:592859/1‑69 (MQ=255)caGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg > 1:451537/1‑69 (MQ=255) aGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAg < 1:804650/68‑1 (MQ=255) gCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCgcggcg > 1:1385272/1‑69 (MQ=255) gCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCgcggcg > 1:1749076/1‑69 (MQ=255) aaTCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGtt > 1:17862/1‑69 (MQ=255) atcatGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCAcgcg > 1:1056128/1‑56 (MQ=255) atcatGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTcc > 1:2287920/1‑69 (MQ=255) atcatGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTcc > 1:511671/1‑69 (MQ=255) atcatGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTcc > 1:1832696/1‑69 (MQ=255) atcatGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTcc > 1:600127/1‑69 (MQ=255) atcatGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTcc > 1:2523096/1‑69 (MQ=255) cgcTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAGCCCGCGCGCGGCGTTTTCCa > 1:3310202/1‑64 (MQ=255) cgcTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAGCACGCGCGCGGCGTTTTCCAGcccc > 1:2293539/1‑69 (MQ=255) cgcTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAGCACGCGCGCGGCGTTTTCCAGcccc > 1:2170482/1‑69 (MQ=255) cgcTGCAACATGCCGCCGCTCATCTCGAACGGGTAGAGCTTCAGCACGCGCGCGGCGTTTTCCAGcccc > 1:2779066/1‑69 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:516312/69‑1 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:1150459/69‑1 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:1309723/69‑1 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:634025/69‑1 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:1489144/69‑1 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:2551121/69‑1 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:247368/69‑1 (MQ=255) cATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTc < 1:2382911/69‑1 (MQ=255) cATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:2739045/69‑1 (MQ=255) cATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:2833895/69‑1 (MQ=255) cATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:1378773/69‑1 (MQ=255) cATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:1577483/69‑1 (MQ=255) cATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:1612181/69‑1 (MQ=255) cATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:1243921/69‑1 (MQ=255) cATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:1958856/69‑1 (MQ=255) ctcGAACGGGTACAGCTTCAACACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt > 1:944853/1‑66 (MQ=255) tcGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAag > 1:918578/1‑68 (MQ=255) tcGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAag > 1:1100577/1‑68 (MQ=255) tcGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAag > 1:2808952/1‑68 (MQ=255) tcGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAag > 1:2372009/1‑68 (MQ=255) tcGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAag > 1:2035411/1‑68 (MQ=255) tcGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAag > 1:199757/1‑68 (MQ=255) aCGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAagcgt < 1:3401189/67‑1 (MQ=255) cGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAagcgtagc < 1:347131/69‑1 (MQ=255) cGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAagcgtagc < 1:335903/69‑1 (MQ=255) cGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAagcgtagc < 1:2535770/69‑1 (MQ=255) cGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAagcgtagc < 1:94820/69‑1 (MQ=255) cAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGt < 1:2954426/54‑1 (MQ=255) | CAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGC > minE/2609707‑2609829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |