| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,612,473 | C→T | 50.0% | A350A (GCG→GCA) | nikA ← | nickel transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,612,473 | 0 | C | T | 50.0% | ‑5.5 / 30.2 | 36 | A350A (GCG→GCA) | nikA | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (4/14); new base T (12/6); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
GGTGCCGATGAACGAAAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGT > minE/2612410‑2612539 | ggTGCCGATGAACGAAAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCAGATGTCTTTGCCCgca > 1:2901548/1‑67 (MQ=255) tGAACGACAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCTGCAGGCAGCGTcc < 1:1421403/69‑1 (MQ=255) tGAACGACAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCTGCAGGCAGCGTcc < 1:361661/69‑1 (MQ=255) tGAACGACAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCTGCAGGCAGCGTcc < 1:3338818/69‑1 (MQ=255) tGAACGACAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCTGCAGGCAGCGTcc < 1:2495619/69‑1 (MQ=255) tGAACGACAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCTGCAGGCAGCGTcc < 1:2381192/69‑1 (MQ=255) tGAACGACAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCTGCAGGCAGCGTcc < 1:2290747/69‑1 (MQ=255) cgcAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAg > 1:108924/1‑68 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCCGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:2938125/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCttttt > 1:2002779/1‑47 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:1106230/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:754800/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:3342164/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:2943860/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:2284618/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:2283169/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:2041695/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgctttc > 1:1979977/1‑61 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAAcgcttt > 1:3185878/1‑60 (MQ=255) tttCTCGCGGATGTCTTTGCCTGCAGGCAGCGTCCAACCGGCTTTTTCCAG‑AAcgctttc > 1:3286695/1‑60 (MQ=255) ttCTCGCGGATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTg > 1:2166782/1‑68 (MQ=255) ttCTCGCGGATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTg > 1:373092/1‑68 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:2196074/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:917837/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:829092/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:61142/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:1615995/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:1658566/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:309917/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:281501/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:2701529/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:2096235/69‑1 (MQ=255) ggATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:2214009/69‑1 (MQ=255) ggATGGCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:1353190/69‑1 (MQ=255) gATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:3108074/68‑1 (MQ=255) gATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGt < 1:568074/68‑1 (MQ=255) ccGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGt < 1:1682460/68‑1 (MQ=255) ccGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGt < 1:1832337/68‑1 (MQ=255) ccGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGt < 1:2145660/68‑1 (MQ=255) | GGTGCCGATGAACGAAAGTTCAATGCGCAGCGGCTGACCATTTTTCTCGCGGATGTCTTTGCCCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGT > minE/2612410‑2612539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |