| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,612,535 | T→G | 68.2% | S330R (AGC→CGC) | nikA ← | nickel transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,612,535 | 0 | T | G | 68.2% | 56.5 / 26.1 | 47 | S330R (AGC→CGC) | nikA | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (6/9); new base G (13/19); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCAAACAGGGTGTCGGCGACCTGCTGGG > minE/2612472‑2612600 | ccGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGt < 1:2145660/68‑1 (MQ=255)ccGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGt < 1:1832337/68‑1 (MQ=255)ccGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGt < 1:1682460/68‑1 (MQ=255) gcaggcagCGTCCAAGCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGGGCGGTTTc > 1:3191388/1‑69 (MQ=255) gcaggcagCGTCCAACGGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:2221791/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGt > 1:1213528/1‑66 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:2218300/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:778699/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:360879/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:3195300/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:1905913/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:1157630/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:1247040/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:1196242/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:1547463/1‑69 (MQ=255) gcaggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTCTGCGGATCGTACTGGCGCGGTTTc > 1:1564594/1‑69 (MQ=255) aggcagCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAg < 1:1610148/69‑1 (MQ=255) ccAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGtt < 1:3043251/69‑1 (MQ=255) ccAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGtt < 1:3189841/69‑1 (MQ=255) ccAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGtt < 1:2534058/69‑1 (MQ=255) cAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTg > 1:1842685/1‑69 (MQ=255) cAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTg > 1:490485/1‑69 (MQ=255) cAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTg > 1:1954873/1‑69 (MQ=255) cAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTg > 1:665046/1‑69 (MQ=255) ccGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTGGCg < 1:3372116/69‑1 (MQ=255) ccGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTGGCg < 1:643296/69‑1 (MQ=255) gCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTg > 1:232734/1‑63 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGTCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:1076913/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:665753/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2694327/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:57122/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:1353337/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:3080332/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2937715/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2891121/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2772925/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2768722/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2460360/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:138524/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2197618/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:2152065/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:1539259/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:1853269/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:1567744/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:1672587/69‑1 (MQ=255) gctttcgctttcTGCGGATCGTACTGGCGCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCa < 1:1614356/69‑1 (MQ=255) ggCTCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCAAACAGGGTGTCTGCGACCTGCTggg > 1:2556787/1‑69 (MQ=255) | CCGCAGGCAGCGTCCAACCGGCTTTTTCCAGCAACGCTTTCGCTTTTTGCGGATCGTACTGGCTCGGTTTCAGGCCGAGGTTGGCGTAGGGCACAGAAGGGGCAAACAGGGTGTCGGCGACCTGCTGGG > minE/2612472‑2612600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |