| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,613,717 | A→T | 67.4% | L168L (CTT→CTA) | acpT ← | holo‑(acyl carrier protein) synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,613,717 | 0 | A | T | 67.4% | 47.7 / 33.8 | 49 | L168L (CTT→CTA) | acpT | holo‑(acyl carrier protein) synthase 2 |
| Reads supporting (aligned to +/- strand): ref base A (12/4); new base T (17/16); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
GCACACTGTCGGCGGTGAGCGTAAAGGGAGTAGGGGTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTT > minE/2613656‑2613775 | gCACACTGTCGGCGGTGAGCGTAAAGGGAGTAGGGGTGCAGATCGCAAGGCTTAAATTTTCAAGCTGAc < 1:1407973/69‑1 (MQ=255) ggcggTGAGCGTAAAGGGAGTAGGGGTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGctgac > 1:1450313/1‑69 (MQ=255) ggcggTGAGCGTAAAGGGAGTAGGGGTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGctga > 1:3292345/1‑68 (MQ=255) ggcggTGAGCGTAAAGGGAGTAGGGGTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGctga > 1:584077/1‑68 (MQ=255) ggcggTGAGCGTAAAGGGAGTAGGGGTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGctga > 1:1870176/1‑68 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGATGAGTTAt < 1:873234/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:243486/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:554677/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2514335/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:543717/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:1878000/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:737426/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:1686133/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:165484/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2784553/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:767860/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:411761/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2942131/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:3329023/61‑1 (MQ=255) gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:3221201/61‑1 (MQ=255) tCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGa < 1:2690050/53‑1 (MQ=255) tCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGa < 1:2081500/53‑1 (MQ=255) tCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGa < 1:1393184/53‑1 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:2288252/1‑69 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:818802/1‑69 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:1170432/1‑69 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:3259192/1‑69 (MQ=255) gCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAt > 1:431886/1‑69 (MQ=255) gCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAt > 1:1007216/1‑69 (MQ=255) cTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAtt > 1:1963073/1‑69 (MQ=255) cTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAtt > 1:1506480/1‑69 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:634831/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:975973/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:3344430/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:831429/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:3100770/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:3005810/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2854121/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2839694/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:275996/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2274556/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2168807/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1769591/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1430063/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1337807/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:13001/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1143681/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1079986/1‑67 (MQ=255) aTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2164811/41‑1 (MQ=255) | GCACACTGTCGGCGGTGAGCGTAAAGGGAGTAGGGGTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTT > minE/2613656‑2613775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |