| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,613,749 | G→T | 73.1% | H158N (CAC→AAC) | acpT ← | holo‑(acyl carrier protein) synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,613,749 | 0 | G | T | 73.1% | 107.4 / 50.7 | 89 | H158N (CAC→AAC) | acpT | holo‑(acyl carrier protein) synthase 2 |
| Reads supporting (aligned to +/- strand): ref base G (11/13); new base T (17/48); total (28/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGCTTC > minE/2613691‑2613812 | gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGATGAGTTAt < 1:873234/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:1878000/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:243486/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2942131/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:3221201/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:767860/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:737426/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2514335/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:554677/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:543717/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:1686133/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:165484/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:411761/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:3329023/61‑1 (MQ=255)gTGCAGATCGCCAGGCTTAAATTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2784553/61‑1 (MQ=255) tCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGa < 1:1393184/53‑1 (MQ=255) tCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGa < 1:2690050/53‑1 (MQ=255) tCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGa < 1:2081500/53‑1 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:3259192/1‑69 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:1170432/1‑69 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:818802/1‑69 (MQ=255) cGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCt > 1:2288252/1‑69 (MQ=255) gCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAt > 1:1007216/1‑69 (MQ=255) gCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAt > 1:431886/1‑69 (MQ=255) cTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAtt > 1:1963073/1‑69 (MQ=255) cTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGAtt > 1:1506480/1‑69 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:3005810/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:3100770/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2854121/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2839694/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:3344430/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:275996/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:634831/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:831429/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:975973/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2274556/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:2168807/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1079986/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1143681/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1430063/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1769591/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:13001/1‑67 (MQ=255) aaaTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGAttt > 1:1337807/1‑67 (MQ=255) aTTTTCTAGCTGACAATGGCTGACTGACAGCGAGGAGTTAt < 1:2164811/41‑1 (MQ=255) tGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACg > 1:1369513/1‑68 (MQ=255) tGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGc > 1:909418/1‑69 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:1830901/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:376185/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:3066082/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:3065751/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:1832917/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:1912295/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:1952823/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:279002/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:1286203/69‑1 (MQ=255) ggCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCt < 1:815039/69‑1 (MQ=255) gCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTg > 1:879636/1‑69 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:3370202/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:1627164/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:1077460/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:331157/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:539314/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:719111/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:1111738/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:2207075/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:244357/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:2094484/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:2611444/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:1770549/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:274432/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:2021395/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:2948230/69‑1 (MQ=255) gacAGCGAGGAGTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACg < 1:1881450/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCTCCACGCTGTTTAACGATGGcttc < 1:2262159/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCATGCTGTTTAACGATGGcttc < 1:3275711/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:482464/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:3081745/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:692466/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:2035828/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:2622257/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:1188928/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:3268271/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:1645777/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:2383412/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:2172589/69‑1 (MQ=255) ggagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:3306709/69‑1 (MQ=255) gagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:1450942/68‑1 (MQ=255) gagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:2026728/68‑1 (MQ=255) gagTTATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGcttc < 1:2339899/68‑1 (MQ=255) | GTGCAGATCGCAAGGCTTAAATTTTCAAGCTGACAATGGCTGACTGACAGCGAGGAGTGATAGGTGCTGTCTACGCTGACGATTTGCCAGGCGCTGCCGCCACGCTGTTTAACGATGGCTTC > minE/2613691‑2613812 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |