| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,614,173 | T→A | 44.9% | P16P (CCA→CCT) | acpT ← | holo‑(acyl carrier protein) synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,614,173 | 0 | T | A | 44.9% | 9.3 / 51.6 | 49 | P16P (CCA→CCT) | acpT | holo‑(acyl carrier protein) synthase 2 |
| Reads supporting (aligned to +/- strand): ref base T (9/18); new base A (5/17); total (14/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
AGCCAGCGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGTT > minE/2614117‑2614235 | agccagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTgcgc > 1:2954287/1‑67 (MQ=255)agccagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCtt < 1:583363/69‑1 (MQ=255)agccagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCtt < 1:1983862/69‑1 (MQ=255)agccagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCtt < 1:1875297/69‑1 (MQ=255) gccagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCtt < 1:2877971/68‑1 (MQ=255) cagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAg < 1:2706233/69‑1 (MQ=255) cagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAg < 1:465514/69‑1 (MQ=255) cagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAg < 1:1317577/69‑1 (MQ=255) cagcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAg < 1:1638795/69‑1 (MQ=255) gcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAgg > 1:1010191/1‑68 (MQ=255) gcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAgg > 1:2466171/1‑68 (MQ=255) gcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAgg > 1:2155192/1‑68 (MQ=255) gcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAgg > 1:3281841/1‑68 (MQ=255) gcGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAgg > 1:384660/1‑68 (MQ=255) tCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGaa < 1:1556868/69‑1 (MQ=255) tCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGaa < 1:435314/69‑1 (MQ=255) aCCTTGCGGTGCTTGCTCGCGTAAACCCGGTGTCAGAGGAGCTGCGCTTAAGGTCGAAACt > 1:1729889/1‑61 (MQ=255) aCCTTGCGGTGCTTGCTCGCGTAAACCCGGTGTCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAg > 1:1506589/1‑69 (MQ=255) aCCTTGCGGTGCTTGCTCGCGTAAACCCGGTGTCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAg > 1:2995837/1‑69 (MQ=255) aCCTTGCGGTGCTTGCTCGCGTAAACCCGGTGTCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAg > 1:1079680/1‑69 (MQ=255) aCCTTGCGGTGCTTGCTCGCGTAAACCCGGTGTCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAg > 1:2471299/1‑69 (MQ=255) tGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACt > 1:2698872/1‑69 (MQ=255) tGCGGTGCTTGCTCGCGTAAAACCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACt > 1:2310567/1‑69 (MQ=255) gTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATcc < 1:3154167/69‑1 (MQ=255) gTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATcc < 1:2398280/69‑1 (MQ=255) gTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATcc < 1:2476809/69‑1 (MQ=255) tgctCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATa > 1:387409/1‑68 (MQ=255) tgctCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATa < 1:512982/68‑1 (MQ=255) tgctCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATa < 1:1038753/68‑1 (MQ=255) tgctCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATa < 1:1042624/68‑1 (MQ=255) tgctCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATa < 1:1044229/68‑1 (MQ=255) tgctCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATa < 1:117118/68‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:884017/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:2772470/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:83499/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:825482/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:636985/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:1061161/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:1154100/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:1524513/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:3293310/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:1678982/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:3167518/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:2033972/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:2142795/69‑1 (MQ=255) tGGCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:2330691/69‑1 (MQ=255) ggCAGAGGAGCTGCGGTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:3086485/68‑1 (MQ=255) ggCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:495065/68‑1 (MQ=255) ggCAGAGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGtt < 1:1884558/68‑1 (MQ=255) | AGCCAGCGTTCGCGTCGTGGACCTTGCGGTGCTTGCTCGCGTAAACCCGGTGGCAGTGGAGCTGCGCTTAAGGTCGAAACTTTCCCCAGAACTATCCGATACATATCAGGGCCAACGTT > minE/2614117‑2614235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |