| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 223,934 | G→A | 60.7% | G387G (GGC→GGT) | pepD ← | aminoacyl‑histidine dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 223,934 | 0 | G | A | 60.7% | 22.2 / 36.6 | 56 | G387G (GGC→GGT) | pepD | aminoacyl‑histidine dipeptidase |
| Reads supporting (aligned to +/- strand): ref base G (10/12); new base A (16/18); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
TGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGAT > minE/223869‑223993 | tGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgcca > 1:3127103/1‑69 (MQ=255)tGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgcc > 1:1815211/1‑68 (MQ=255) ggAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTa < 1:2565878/69‑1 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa < 1:893389/69‑1 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa > 1:421904/1‑69 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa > 1:3369392/1‑69 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa > 1:1953049/1‑69 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa > 1:1918986/1‑69 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa > 1:1890195/1‑69 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa > 1:1833307/1‑69 (MQ=255) aGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGa > 1:1697279/1‑69 (MQ=255) aGCGTCCGGCTGACAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCTGTTTACCCAGCGa > 1:107000/1‑69 (MQ=255) cGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAg < 1:2630829/69‑1 (MQ=255) cGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAg < 1:3388135/69‑1 (MQ=255) tGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATg < 1:286331/69‑1 (MQ=255) tGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATg < 1:1025663/69‑1 (MQ=255) tGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATg < 1:319156/69‑1 (MQ=255) gccagccaGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATg < 1:1450951/68‑1 (MQ=255) agccagGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTca < 1:2182369/69‑1 (MQ=255) agccagGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTca < 1:2178518/69‑1 (MQ=255) cTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTAc > 1:2592032/1‑69 (MQ=255) cTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTAc > 1:2558478/1‑69 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:2992747/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:3319319/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:2866231/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:3391807/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:3435200/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:414526/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:1572751/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:2546679/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:2495377/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:870780/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:1292815/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:193818/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:1310975/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:183462/3‑68 (MQ=255) ctgTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:1322440/3‑68 (MQ=255) ctgTTTTCGCACCTGACAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCTTTACCGCTGTCg > 1:2328473/3‑68 (MQ=255) gTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGAt < 1:762993/68‑1 (MQ=255) gTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGAt < 1:603652/68‑1 (MQ=255) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:1004121/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:728164/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:1008461/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:1595574/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:875990/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:423930/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:501357/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:635133/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:389418/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:331761/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:3163756/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:2912181/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:2904239/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:1251312/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:2562617/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:2120147/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:1893660/40‑1 (MQ=37) ttttCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCt < 1:1536991/40‑1 (MQ=37) | TGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGAT > minE/223869‑223993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |