| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,616,025 | G→A | 100% | A190A (GCG→GCA) | yhhS → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,616,025 | 0 | G | A | 83.5% | 13.8 / ‑2.7 | 12 | A190A (GCG→GCA) | yhhS | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (10/0); total (12/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTTTATCACTGGGGCGGCTTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCGCTGGTGGCCATTTTGTTGGCGATCCCGCGTCCGACGGTAAAA > minE/2615971‑2616067 | gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:1027300/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:1507108/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:2255340/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:2288668/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:2907890/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:2915350/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:3135402/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:3207889/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:3428616/1‑69 (MQ=255)gtTTTATCACTGGGGCGGATTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCACTGGTGGCCAtttt > 1:543036/1‑69 (MQ=255) ggcggcTTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCGCTGGTGGCCATTTTGTTGGCGATCCcg > 1:3293462/1‑69 (MQ=255) agcgttaATCATTATGGGCGTGGCGCTGGTGGCCATTTTGTTGGCGATCCCGCGTCCGACGGTaaaa > 1:2515752/1‑67 (MQ=255) | GTTTTATCACTGGGGCGGCTTGCAGGCGTTAGCGTTAATCATTATGGGCGTGGCGCTGGTGGCCATTTTGTTGGCGATCCCGCGTCCGACGGTAAAA > minE/2615971‑2616067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |