BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,616,163	C→T	37.0%	V236V (GTC→GTT)	yhhS →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,616,163	0	C	T	37.0%	36.6 / 36.2	62	V236V (GTC→GTT)	yhhS	predicted transporter
Reads supporting (aligned to +/- strand): ref base C (28/11); new base T (9/14); total (37/25)
Fisher's exact test for biased strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTC  >  minE/2616101‑2616220

gTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGcc                                                     <  1:968954/69‑1 (MQ=255)

gTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGcc                                                     <  1:482892/69‑1 (MQ=255)

gTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGcc                                                     <  1:3399756/69‑1 (MQ=255)

gTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGcc                                                     <  1:3221591/69‑1 (MQ=255)

gTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGcc                                                     <  1:2648834/69‑1 (MQ=255)

gTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGcc                                                     <  1:143806/69‑1 (MQ=255)

         cgcgTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCGTCGCCACCTTTAt                                             <  1:2502305/68‑1 (MQ=255)

         cgcgTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTAt                                             <  1:381517/68‑1 (MQ=255)

                       cGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATg                               >  1:3373616/1‑68 (MQ=255)

                       cGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGa                              >  1:749166/1‑69 (MQ=255)

                       cGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGa                              <  1:473214/69‑1 (MQ=255)

                       cGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGa                              >  1:2498317/1‑69 (MQ=255)

                       cGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGa                              >  1:361145/1‑69 (MQ=255)

                       cGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGa                              >  1:18072/1‑69 (MQ=255)

                       cGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGa                              >  1:2629840/1‑69 (MQ=255)

                              gcgcTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTaa                        <  1:2305823/68‑1 (MQ=255)

                              gcgcTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTaa                        >  1:2403638/1‑68 (MQ=255)

                              gcgcTGGCACTGGCTTCCGACGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTaa                        <  1:2362430/68‑1 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGGCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:3281179/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAgg                     >  1:1581339/1‑60 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:3181242/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:2946465/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:2688802/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:2452770/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:460276/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:487236/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:1300969/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:1452416/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:2201522/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:2195152/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:1270388/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACgg            >  1:1961441/1‑69 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACg             >  1:117991/1‑68 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACg             >  1:1933588/1‑68 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACg             >  1:286372/1‑68 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACg             >  1:3134655/1‑68 (MQ=255)

                                         ggCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGATGTTTTATGACGCTAAAGGTTGGGACgg            >  1:743688/1‑69 (MQ=255)

                                          gCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGt           >  1:1795963/1‑69 (MQ=255)

                                          gCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGt           >  1:1993656/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:1310633/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:71665/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:112810/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:1936280/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:74550/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:2732315/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:1005195/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCtt    >  1:861409/1‑69 (MQ=255)

                                                 ccgGATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCt     >  1:1238559/1‑68 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:879038/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:899213/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:630591/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:1057241/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:1107984/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:1495939/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:3250435/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:1164602/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:2750703/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:2220650/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:226507/69‑1 (MQ=255)

                                                   ggATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:2442934/69‑1 (MQ=255)

                                                    gATTTGGCGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:467656/68‑1 (MQ=255)

                                                           cGTTATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTc  <  1:2303485/61‑1 (MQ=255)

GTGCTTGGGCGCGTCTGGCTGTACGGTATGGCGCTGGCACTGGCTTCCGCCGGATTTGGCGTCATCGCCACCTTTATCACGCTGTTTTATGACGCTAAAGGTTGGGACGGTGCGGCTTTC  >  minE/2616101‑2616220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`