| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,617,691 | G→T | 32.0% | S94S (TCC→TCA) | yhhQ ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,617,691 | 0 | G | T | 32.0% | 121.8 / 63.4 | 100 | S94S (TCC→TCA) | yhhQ | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (23/45); new base T (17/15); total (40/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TGAAACTGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGA > minE/2617624‑2617758 | tGAAACTGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAgg < 1:2488903/69‑1 (MQ=255) tGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCa < 1:1069378/69‑1 (MQ=255) tGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCa < 1:3295755/69‑1 (MQ=255) tGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCa < 1:3241249/69‑1 (MQ=255) ggTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCata < 1:232170/67‑1 (MQ=255) gTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAg < 1:1020359/69‑1 (MQ=255) gTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAg < 1:1676501/69‑1 (MQ=255) gTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAg < 1:3219115/69‑1 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2758470/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2950630/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2981795/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2455131/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:376334/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2223850/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:2060895/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1953119/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:585023/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:3208974/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1506173/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1209263/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:1353402/62‑2 (MQ=255) tACGGGCGACAAACAGGTTGAAGTGGGCGAGCGCGCCGAATCCTTGCCATGACCCCAtataa < 1:135644/62‑2 (MQ=255) gctaCAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:300215/66‑1 (MQ=255) gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:642899/69‑1 (MQ=255) gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:35063/69‑1 (MQ=255) gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:2367590/69‑1 (MQ=255) gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:33337/69‑1 (MQ=255) gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:2905312/69‑1 (MQ=255) gCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:910197/69‑1 (MQ=255) cGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:536454/68‑1 (MQ=255) cGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACgag < 1:1229325/68‑1 (MQ=255) gTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:1143512/69‑1 (MQ=255) gTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:1664173/69‑1 (MQ=255) gTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2249286/69‑1 (MQ=255) gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:58399/49‑1 (MQ=255) gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:703543/49‑1 (MQ=255) gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:684833/49‑1 (MQ=255) gAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGc < 1:2360554/49‑1 (MQ=255) tGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2590432/62‑1 (MQ=255) ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGCATAGCGACGAGATGACGTAGga < 1:3142348/60‑1 (MQ=255) ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2974658/60‑1 (MQ=255) ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:3090544/60‑1 (MQ=255) ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:3376493/60‑1 (MQ=255) ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:171409/60‑1 (MQ=255) ggCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:450812/60‑1 (MQ=255) ggCGACTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:2453329/60‑1 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGga < 1:3153321/54‑1 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:82201/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:3072595/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:612177/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:1992714/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:617806/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:2594126/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:2231138/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCa > 1:1474799/1‑67 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAgg > 1:2576192/1‑69 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAgg > 1:1253656/1‑69 (MQ=255) tGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAgg > 1:1979635/1‑69 (MQ=255) ccGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGAt < 1:1598137/68‑1 (MQ=255) ccGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGAt < 1:2829793/68‑1 (MQ=255) ccGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGAt < 1:1236117/68‑1 (MQ=255) aaTCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAtt < 1:2083190/69‑1 (MQ=255) aaTCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAtt < 1:1255979/69‑1 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGGAATGCGGGGATCa > 1:1429148/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATc > 1:310434/1‑64 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:858748/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:678474/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:325766/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:3372276/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:10266/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1072707/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1110254/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1128437/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1146064/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:2705635/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1190698/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:2520323/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:1891244/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:190258/1‑65 (MQ=255) tCCTTGCCATGACCCCATATAAAACAGCGATGAGATGACGTAGGAGATCAGCAATGCGGGGATCa > 1:3206630/1‑65 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACgaga > 1:1457972/1‑35 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTa > 1:2442406/1‑68 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:2169768/1‑69 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:1629162/1‑69 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:165292/1‑69 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:3316298/1‑69 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:2840864/1‑69 (MQ=255) tCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTAc > 1:3204965/1‑69 (MQ=255) ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:533646/1‑64 (MQ=255) ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:2421347/1‑64 (MQ=255) ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:356670/1‑64 (MQ=255) ccTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCAt > 1:702083/1‑64 (MQ=255) aGGAACCCATATAGAATATCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:614695/69‑1 (MQ=255) aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATCACGCAGGGATCATTACCGCGAAGa < 1:3235735/69‑1 (MQ=255) aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2431780/69‑1 (MQ=255) aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:628811/69‑1 (MQ=255) aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:1562420/69‑1 (MQ=255) aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2576512/69‑1 (MQ=255) aGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2789802/69‑1 (MQ=255) ggAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGa < 1:2178731/68‑1 (MQ=255) | TGAAACTGGCGGTGGCGATACGGGCGACAAACAGGTTGAAGTGGGCGAGTGCGCCGAATCCCTGCCAGGAACCCATATAGAATAGCGACGAGATGACGTAGGAGATTAATAACGCAGGGATCATTACCGCGAAGA > minE/2617624‑2617758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |