| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,619,677 | A→G | 57.9% | Y354Y (TAT→TAC) | zntA ← | zinc, cobalt and lead efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,619,677 | 0 | A | G | 57.9% | 20.5 / 45.1 | 64 | Y354Y (TAT→TAC) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base A (12/15); new base G (31/6); total (43/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
TGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCT > minE/2619613‑2619734 | tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:19884/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:940490/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:846274/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:1174669/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:2207471/69‑1 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:2211539/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:2815054/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:3213286/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:1607366/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:592156/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:135403/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:3202302/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:1277104/69‑1 (MQ=255) cACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:209127/68‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:1403107/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:3069154/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:2477753/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:1094997/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:2481791/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:913888/69‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:3066179/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:1995341/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:153234/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:1396610/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:2458816/43‑1 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2763744/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2478615/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2358850/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2345198/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2321372/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2181769/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:327510/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:1274166/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:1068341/3‑67 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCaa > 1:2386833/2‑68 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:3102736/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:378010/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:468730/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2985705/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2904186/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:534268/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:578750/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:71371/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2562213/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:814914/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:240914/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:239925/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2225678/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:22133/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1747421/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1559124/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1379873/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1324010/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1142794/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:958432/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGATCAAt > 1:981530/2‑69 (MQ=255) aTCGCGGGCGTATAGATACTGCTGAAACGGTCGATAAACCGCTCAATGGCAg > 1:2145346/1‑52 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:3402567/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:2592990/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:1946408/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:1066069/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGct > 1:2494269/1‑69 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGc > 1:1045715/1‑68 (MQ=255) aTCGAGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:2217670/1‑60 (MQ=255) | TGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCT > minE/2619613‑2619734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |