| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,619,680 | G→A | 39.7% | I353I (ATC→ATT) | zntA ← | zinc, cobalt and lead efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,619,680 | 0 | G | A | 39.7% | 46.5 / 62.0 | 93 | I353I (ATC→ATT) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base G (27/29); new base A (31/6); total (58/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGTT > minE/2619613‑2619747 | tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:940490/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:19884/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:846274/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:2211539/69‑1 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa > 1:1174669/1‑69 (MQ=255)tGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:2207471/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:1277104/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:2815054/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:3213286/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:3202302/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:135403/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:592156/69‑1 (MQ=255) gCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:1607366/69‑1 (MQ=255) cACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATaa < 1:209127/68‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:2481791/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:3069154/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:1094997/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:1403107/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:913888/69‑1 (MQ=255) cAGCGTTACCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCg < 1:2477753/69‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:3066179/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:153234/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:1396610/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:1995341/43‑1 (MQ=255) aGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGa < 1:2458816/43‑1 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2763744/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2478615/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2321372/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:327510/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2358850/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2345198/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:2181769/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:1274166/3‑67 (MQ=255) ttaCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTc > 1:1068341/3‑67 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCaa > 1:2386833/2‑68 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:240914/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:239925/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:3102736/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2562213/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2904186/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2985705/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:2225678/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:378010/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:468730/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:534268/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:578750/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:71371/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:814914/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:958432/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1142794/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:22133/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1324010/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1379873/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1559124/2‑69 (MQ=255) taCCAGCAGGGCGACGGCCATAATCGCTGGCGTGTAAATACGGCTGAAACGGTCGATAAACCGCTCAAt > 1:1747421/2‑69 (MQ=255) aTCGCGGGCGTATAGATACTGCTGAAACGGTCGATAAACCGCTCAATGGCAg > 1:2145346/1‑52 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:3402567/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:1946408/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:2592990/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:1066069/1‑60 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGct > 1:2494269/1‑69 (MQ=255) aTCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGc > 1:1045715/1‑68 (MQ=255) aTCGAGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGc > 1:2217670/1‑60 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGGGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:1399189/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCTCGACGCTCTTCGGCTTCTTCGATc < 1:1085747/66‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATc < 1:515307/66‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATc < 1:2053452/66‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATc < 1:3232107/66‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATc < 1:2376719/66‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATc < 1:2054912/66‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAg > 1:1478339/1‑68 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAg > 1:1585597/1‑68 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:606678/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt < 1:1356699/69‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:3269856/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt < 1:131902/69‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt < 1:333638/69‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:74540/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:809476/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt < 1:1534725/69‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:3262884/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:1560508/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:1596296/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt < 1:2134562/69‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:2977207/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:1878254/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:1935434/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt < 1:2108503/69‑1 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:263052/1‑69 (MQ=255) taGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:2074063/1‑69 (MQ=255) taGATACGGCTGAAACGGGCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATc < 1:217469/66‑1 (MQ=255) aGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt < 1:1663742/68‑1 (MQ=255) gATACGGCTGAAACGGTCGATAACCCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGtt > 1:942242/1‑68 (MQ=255) gATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAg > 1:2372738/1‑66 (MQ=255) gATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:1045135/1‑67 (MQ=255) gATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:689790/1‑67 (MQ=255) gATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:2038740/1‑67 (MQ=255) gATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:2045592/1‑67 (MQ=255) gATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:2146700/1‑67 (MQ=255) gATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGt > 1:102329/1‑67 (MQ=255) | TGGCGGCAAACAGCAGCGGTGGCACCAGCGTCACCAGCAGAGCGACGGCCATAATCGCGGGCGTATAGATACGGCTGAAACGGTCGATAAACCGCTCAATGGGAGCGCGACGCTCTTCGGCTTCTTCGATCAGTT > minE/2619613‑2619747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |