Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,620,337 | T→A | 35.7% | L134L (CTA→CTT) | zntA ← | zinc, cobalt and lead efflux system |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,620,337 | 0 | T | A | 35.7% | 83.9 / 77.1 | 95 | L134L (CTA→CTT) | zntA | zinc, cobalt and lead efflux system |
Reads supporting (aligned to +/- strand): ref base T (47/14); new base A (19/15); total (66/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.86e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 |
AACGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCA > minE/2620272‑2620400 | aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGCCCCCAGCTGATTGCCATCATCACGATTAg > 1:3355206/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTa > 1:3287456/1‑67 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2093967/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3168259/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3089738/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3000377/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:3301573/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:341299/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:224054/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2058161/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:528100/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2014849/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:598314/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:1587299/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:1269234/1‑68 (MQ=255) aaCGCCAGTTGCCCGAACGGATGATTGAACTGCTCAAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:733713/1‑68 (MQ=255) aaCGCCAGTTGCCCCAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAg > 1:2252322/1‑68 (MQ=255) cGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt < 1:2081111/69‑1 (MQ=255) gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:2414297/69‑1 (MQ=255) gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:2368494/69‑1 (MQ=255) gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:1004543/69‑1 (MQ=255) gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:3005268/69‑1 (MQ=255) gCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTa < 1:830944/69‑1 (MQ=255) gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt > 1:2226627/1‑61 (MQ=255) gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt > 1:2824499/1‑61 (MQ=255) gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGt > 1:1842910/1‑61 (MQ=255) gCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCAACACGAtt > 1:1993048/1‑56 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:3311995/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2669697/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2957655/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1668761/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:174241/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2707514/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:272143/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2234014/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1576992/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1175781/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:2791877/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1576279/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:1392888/57‑1 (MQ=255) aaaCGGATGATTGAACTGCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:908439/57‑1 (MQ=255) aaaCGGATGATTGAACTCCTCCAGCCCCCAACTGATTGCCATCATCACGATAAGCGTa < 1:3266032/57‑1 (MQ=37) cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCa > 1:442350/1‑59 (MQ=255) cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAtt > 1:843288/1‑69 (MQ=255) cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAtt > 1:2081798/1‑69 (MQ=255) cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAtt > 1:319026/1‑69 (MQ=255) cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAt > 1:925739/1‑68 (MQ=255) cGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGAt > 1:3381202/1‑68 (MQ=255) tgatTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGAGTAATCAGCGGCAGATtctc > 1:3355191/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCtgcggc < 1:702755/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1244044/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1042423/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:2800136/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1271170/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1325681/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1873377/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCggcggc < 1:1624022/65‑1 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCt > 1:3400343/1‑67 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:3432237/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:429859/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:510822/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:998817/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:761222/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:3065266/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:139569/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:1749609/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2320557/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2323086/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2449545/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:2853280/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:3114968/1‑68 (MQ=255) atcaCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTg > 1:301191/1‑68 (MQ=255) aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATg > 1:1394485/1‑43 (MQ=255) aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCa > 1:2735764/1‑69 (MQ=255) aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCa > 1:1869448/1‑69 (MQ=255) aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCa > 1:588459/1‑69 (MQ=255) aCGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCATGTTCa > 1:2916101/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTgtg > 1:1246175/1‑47 (MQ=38) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGttcg > 1:2461339/1‑53 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:711056/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1110103/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:332859/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:890658/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:870076/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1495998/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:3238426/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1781794/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:371857/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2481014/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:1970568/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2033795/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:515081/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2207966/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:2948386/1‑69 (MQ=255) aCGATAAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTAggaggc > 1:2705350/1‑62 (MQ=255) aCGATAAGCGTAATCAGAGGCAGATTCTCTTTCAGGCGTGATTCTTGTGGTTCGTCGGAGGCCTGTTCa > 1:3010782/1‑69 (MQ=255) | AACGCCAGTTGCCCGAACGGATGATTGAACTGCTCCAGACCCCAGCTGATTGCCATCATCACGATTAGCGTAATCAGCGGCAGATTCTCTTTCAGGCGTGATGCTTGCGGTTCTTCGGCGGCCTGTTCA > minE/2620272‑2620400 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |