| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,623,335 | T→C | 50.7% | A130A (GCT→GCC) | ftsY → | fused Signal Recognition Particle (SRP) receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,623,335 | 0 | T | C | 50.7% | ‑1.1 / 64.9 | 73 | A130A (GCT→GCC) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base T (13/23); new base C (15/22); total (28/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAA > minE/2623271‑2623393 | gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2930981/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2494099/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2224001/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2067477/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2545367/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2030346/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:3367364/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:1977305/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:607582/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2944711/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2653095/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:720852/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:1739485/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:788271/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:828096/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2798502/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:27380/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2453609/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:929604/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2287278/68‑1 (MQ=255)gTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGACAGGCCGaa < 1:610215/68‑1 (MQ=255)gTGAAGAACTGACGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGaa < 1:2972576/68‑1 (MQ=255) gagCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGa < 1:2779809/68‑1 (MQ=255) gagCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGa < 1:750153/68‑1 (MQ=255) gCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAAc > 1:2363530/1‑69 (MQ=255) cTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAAc > 1:1293118/1‑68 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGTAAACCg < 1:2415232/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:2957658/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:3298514/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:2450169/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:2444283/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:3212998/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:2329260/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:2228621/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:213842/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:3311914/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:1905409/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:613070/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:713570/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:882606/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCg < 1:998435/62‑1 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAgaga > 1:862827/1‑68 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:3285323/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:1089636/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:2217968/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:1957240/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:1937349/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:2002024/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:1876939/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:1730379/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:2247107/1‑69 (MQ=255) gCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGAt > 1:2607974/1‑69 (MQ=255) ggTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaagaaga < 1:2752739/66‑1 (MQ=255) gTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCg < 1:723024/69‑1 (MQ=255) gTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCg < 1:285858/69‑1 (MQ=255) gTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCg < 1:563528/69‑1 (MQ=255) gaagaGTGGCAGGCTGAAGCGGAAAGCGTAGAGATTGTCTAAGCGGCGgaagaaga < 1:108021/56‑1 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAgc > 1:1433620/1‑44 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:170671/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:1367695/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:2717843/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:740509/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:1201031/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:690103/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:1899855/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:3431439/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:3350560/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:3330709/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:2467670/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:2521185/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:2709370/1‑52 (MQ=255) gaagaGTGGCAGGCCGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGgaag > 1:2899657/1‑52 (MQ=255) agagTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAagaaga < 1:983262/69‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:553564/59‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:699185/59‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:1892151/59‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:2056709/59‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:3309633/59‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:1747872/59‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:3301301/59‑1 (MQ=255) tGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGaaa < 1:2405026/59‑1 (MQ=255) | GTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAACCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAA > minE/2623271‑2623393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |