| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,623,644 | G→A | 42.9% | E233E (GAG→GAA) | ftsY → | fused Signal Recognition Particle (SRP) receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,623,644 | 0 | G | A | 42.9% | 41.6 / 85.0 | 114 | E233E (GAG→GAA) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base G (44/21); new base A (28/21); total (72/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGC > minE/2623585‑2623712 | tttATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:2894371/69‑1 (MQ=255)tttATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:2293831/69‑1 (MQ=255)tttATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:3033761/69‑1 (MQ=255)tttATCAGCCTGTGCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTg < 1:1671254/69‑1 (MQ=255) aGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:463969/69‑1 (MQ=255) aGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:156624/69‑1 (MQ=255) aGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:121917/69‑1 (MQ=255) cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:1438208/1‑69 (MQ=255) cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:2002535/1‑69 (MQ=255) cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:590117/1‑69 (MQ=255) cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:2788213/1‑69 (MQ=255) cTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:1789734/1‑69 (MQ=255) tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGAtgtg > 1:3341415/1‑68 (MQ=255) tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:3061184/1‑69 (MQ=255) tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:1595835/1‑69 (MQ=255) tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:2928785/1‑69 (MQ=255) tCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTgg > 1:437462/1‑69 (MQ=255) aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:225417/1‑53 (MQ=255) aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:1214477/1‑53 (MQ=255) aaaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:842562/1‑53 (MQ=255) aaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGc > 1:2830007/1‑52 (MQ=255) aaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGaa > 1:2977405/1‑68 (MQ=255) aaaaaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATAGCCGATGTGGGTGTGGa > 1:1640572/1‑67 (MQ=255) aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:147726/69‑1 (MQ=255) aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:957601/69‑1 (MQ=255) aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:1973201/69‑1 (MQ=255) aaaTCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACca < 1:236337/69‑1 (MQ=255) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAt > 1:2475052/1‑43 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2678697/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:286605/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2000469/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2845571/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2887329/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2141025/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2072459/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1642928/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:530891/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:988592/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1155659/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1186333/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1249970/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2937253/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1258448/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:524887/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:1569402/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgggcg > 1:2993965/1‑48 (MQ=37) tgatCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCC‑ATGTgggc > 1:1565781/1‑47 (MQ=21) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTTGGTGTGGAAACCACACGTaa > 1:1082915/1‑65 (MQ=255) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:1304813/1‑65 (MQ=255) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:357903/1‑65 (MQ=255) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:2340394/1‑65 (MQ=255) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTaa > 1:2748434/1‑65 (MQ=255) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAAtt < 1:2183212/69‑1 (MQ=255) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAAtt < 1:110895/69‑1 (MQ=255) gatCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAAt > 1:2735477/1‑68 (MQ=255) atCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGcc < 1:1003905/38‑1 (MQ=255) tttGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCAcc > 1:2626072/1‑69 (MQ=255) tttGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCAcc > 1:1742845/1‑69 (MQ=255) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:3122100/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1518068/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:3404661/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1656814/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2995933/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1657870/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1706627/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:3405038/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:203652/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1439706/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2864660/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:471829/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2366974/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2808830/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:125092/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2441016/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:1242001/40‑1 (MQ=25) tttGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTgg < 1:2726344/40‑1 (MQ=25) tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:425164/1‑64 (MQ=255) tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:1001996/1‑64 (MQ=255) tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:235628/1‑64 (MQ=255) tGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATc > 1:1769935/1‑64 (MQ=255) gagCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACTTAAAATTATCACCAATCt < 1:307574/68‑1 (MQ=255) gagCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCt < 1:3141327/68‑1 (MQ=255) gagCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCt < 1:2604227/68‑1 (MQ=255) gagCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCt < 1:2081303/68‑1 (MQ=255) gCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGa > 1:3263775/1‑68 (MQ=255) gCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGa > 1:1210186/1‑68 (MQ=255) gCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGa > 1:818364/1‑68 (MQ=255) gCTGGAACAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGa > 1:1756777/1‑68 (MQ=255) ggAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGa > 1:1481676/1‑36 (MQ=255) ggAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGa > 1:2401100/1‑36 (MQ=255) ggAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGa > 1:1579593/1‑36 (MQ=255) ggAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGa > 1:714075/1‑36 (MQ=255) ggAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGa > 1:3264254/1‑36 (MQ=255) ggAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGa > 1:1020204/1‑36 (MQ=255) ggAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGa > 1:2634925/1‑36 (MQ=255) agcagcTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGgcg < 1:709683/69‑1 (MQ=255) gcagcTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGgcgc > 1:1460880/1‑69 (MQ=255) gcagcTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGgcgc > 1:1367679/1‑69 (MQ=255) gcagcTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGgcgc > 1:759094/1‑69 (MQ=255) gcagcTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGgcgc > 1:3327199/1‑69 (MQ=255) | TTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTGGGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGC > minE/2623585‑2623712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |