| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,624,109 | G→A | 32.7% | Q388Q (CAG→CAA) | ftsY → | fused Signal Recognition Particle (SRP) receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,624,109 | 0 | G | A | 32.7% | 49.6 / 29.2 | 61 | Q388Q (CAG→CAA) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base G (20/21); new base A (9/11); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CAGGCAGCTAAAGCGCGTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGC > minE/2624050‑2624177 | cAGGCAGCTAAAGCGCGTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCg > 1:436797/1‑69 (MQ=255)cAGGCAGCTAAAGCGCGTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCg > 1:373587/1‑69 (MQ=255)cAGGCAGCTAAAGCGCGTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCg > 1:1575538/1‑69 (MQ=255)cAGGCAGCTAAAGCGCGTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCg > 1:1600882/1‑69 (MQ=255) tatCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:2715806/67‑1 (MQ=255) tggACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTgaag > 1:1787864/3‑68 (MQ=255) tCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTgaag > 1:170073/1‑68 (MQ=255) tCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTgaag > 1:3333068/1‑68 (MQ=255) tCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTgaag > 1:2810155/1‑68 (MQ=255) tCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGCAAGAGTTgaa > 1:2564438/1‑67 (MQ=255) gACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:2243010/63‑1 (MQ=255) gACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:2761901/63‑1 (MQ=255) gACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:2356700/63‑1 (MQ=255) gACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:1854364/63‑1 (MQ=255) gACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:3428339/63‑1 (MQ=255) gACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:414359/63‑1 (MQ=255) aCGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTg < 1:1495269/62‑1 (MQ=255) gTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATc > 1:2273921/1‑69 (MQ=255) gTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATc > 1:1887181/1‑69 (MQ=255) gTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATc > 1:3123813/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:2508005/57‑1 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:2147157/57‑1 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:615186/57‑1 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:1698645/57‑1 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:1645272/57‑1 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:876213/57‑1 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:975965/57‑1 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATc > 1:3236790/1‑61 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:423563/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:2213102/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:2576444/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:462058/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:573523/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:2006328/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:797919/1‑69 (MQ=255) tGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGt > 1:1482378/1‑69 (MQ=255) gCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:1592200/56‑1 (MQ=255) gCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:302868/56‑1 (MQ=255) ccGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:686671/55‑1 (MQ=255) ccGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTgaagaa < 1:2730018/55‑1 (MQ=255) accgCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaa > 1:726538/4‑68 (MQ=255) aCAGCCGGCCGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaa > 1:2557939/1‑68 (MQ=255) aCAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaa > 1:3285888/1‑68 (MQ=255) aCAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaa > 1:2597100/1‑68 (MQ=255) aCAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaa > 1:2903809/1‑68 (MQ=255) aCAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaa > 1:13274/1‑68 (MQ=255) aCAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaa > 1:206600/1‑68 (MQ=255) gCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaagaa < 1:3228752/68‑1 (MQ=255) gCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaagaa < 1:2648617/68‑1 (MQ=255) gCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaagaa < 1:707241/68‑1 (MQ=255) gCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATgaagaa < 1:2211580/68‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:3408289/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:1512828/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:1731469/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:1753197/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:1849292/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:208400/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:246503/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:2535894/69‑1 (MQ=255) ggACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:2627251/69‑1 (MQ=255) cAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGaa > 1:477383/1‑69 (MQ=255) gAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTc < 1:2708072/58‑1 (MQ=255) gAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgc > 1:3313716/1‑69 (MQ=255) gAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAgc > 1:1146395/1‑69 (MQ=255) | CAGGCAGCTAAAGCGCGTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGC > minE/2624050‑2624177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |