| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,625,741 | T→C | 61.4% | R213R (CGT→CGC) | ftsX → | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,625,741 | 0 | T | C | 61.4% | 12.5 / 16.5 | 31 | R213R (CGT→CGC) | ftsX | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (6/6); new base C (4/15); total (10/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGATG > minE/2625685‑2625798 | aTCACGCAGATTAACGGCATTGACGAAGTGCGGTTGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:2317545/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:2215603/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:3403959/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:3335084/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:3133115/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:3000810/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:2942215/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:2592946/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:830426/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:842793/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:863742/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:945501/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:1645903/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:1467348/68‑1 (MQ=255)aTCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGtt < 1:1231668/68‑1 (MQ=255) tGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGTTGAc > 1:2934833/1‑51 (MQ=255) tGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGt > 1:1965061/1‑69 (MQ=255) tGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGt > 1:247736/1‑69 (MQ=255) tGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGCCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGt > 1:1838183/1‑69 (MQ=255) gACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGtt > 1:1251578/1‑69 (MQ=255) gACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGtt > 1:566636/1‑69 (MQ=255) gACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGtt > 1:2705348/1‑69 (MQ=255) cTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAt < 1:475582/69‑1 (MQ=255) cTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAt < 1:675416/69‑1 (MQ=255) cTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAt < 1:811561/69‑1 (MQ=255) cTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAt < 1:205243/69‑1 (MQ=255) cTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAt < 1:1793044/69‑1 (MQ=255) cTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAt < 1:1672165/69‑1 (MQ=255) tGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAtg > 1:544639/1‑69 (MQ=255) tGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAtg > 1:2129897/1‑69 (MQ=255) tGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGAtg > 1:978663/1‑69 (MQ=255) | ATCACGCAGATTAACGGCATTGACGAAGTGCGGATGGATGACAGCTGGTTTGCCCGTCTGGCGGCGTTGACCGGGCTGGTCGGGCGCGTTTCGGCGATGATCGGCGTGTTGATG > minE/2625685‑2625798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |