| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,627,456 | T→C | 30.0% | intergenic (+196/‑75) | rpoH → / → livJ | RNA polymerase, sigma 32 (sigma H) factor/leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,627,456 | 0 | T | C | 30.0% | 125.7 / 64.8 | 133 | intergenic (+196/‑75) | rpoH/livJ | RNA polymerase, sigma 32 (sigma H) factor/leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (56/37); new base C (29/11); total (85/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGA > minE/2627391‑2627522 | cTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGc > 1:1828418/1‑68 (MQ=255)cTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGc > 1:1281061/1‑68 (MQ=255)cTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGc > 1:448190/1‑68 (MQ=255) atCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaa > 1:2876972/1‑68 (MQ=255) atCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaa > 1:1287900/1‑68 (MQ=255) atCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaa > 1:1317310/1‑68 (MQ=255) atCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGT‑GTCATGCATAAAACGaaa > 1:1224754/1‑68 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2919187/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2068140/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2287792/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:1699114/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2555497/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2713451/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:876819/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATCCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:1612487/69‑1 (MQ=255) tCATAAAAATGAGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2389189/69‑1 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACg > 1:3366619/1‑65 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:2458237/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:2753797/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:3310123/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:2019809/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:1107181/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:1723230/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:1081325/1‑68 (MQ=255) aaaaTGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:2676513/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:600934/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:3023615/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:2931057/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:1920309/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:1387801/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:221873/1‑69 (MQ=255) aTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGc > 1:2314334/1‑68 (MQ=255) aTGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaac < 1:841115/69‑1 (MQ=255) aTGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaac < 1:1109529/69‑1 (MQ=255) tGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaac > 1:2609688/1‑68 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:859805/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:2688931/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:2807280/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:2862916/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:134756/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:2508800/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:3109676/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:540370/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:2395650/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:500255/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:171611/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:604550/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaa < 1:408182/68‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaa < 1:2001540/68‑1 (MQ=255) tttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:1933772/68‑1 (MQ=255) agagTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCacaa < 1:2450874/69‑1 (MQ=255) agagTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCacaa < 1:2214616/69‑1 (MQ=255) agagTATGCTGCTAAAGCACGGGTACTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCacaa < 1:792363/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:840056/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:2966794/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:2846393/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:538671/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:1172861/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:512482/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:405143/69‑1 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:3399875/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:417262/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:2976067/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:3022089/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:183490/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:3033120/1‑68 (MQ=255) ctgctAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGt < 1:1831317/68‑1 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTg > 1:2963701/1‑36 (MQ=16) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGGACAACAACATCACAacacac > 1:3160496/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACTTCACAacacac > 1:2554691/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAataca > 1:878634/1‑56 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:370838/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:550690/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1777977/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1728420/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1582422/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1499013/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:2539198/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1343352/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:861104/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:2642287/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:2659112/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1130596/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:3286560/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:327869/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:3173566/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:309436/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacaca > 1:2934237/1‑56 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATAACAacacac > 1:3193433/1‑57 (MQ=255) aCGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAacaaca < 1:2439125/45‑1 (MQ=255) ggTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAgaagaa < 1:1119225/69‑1 (MQ=255) ggTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAgaagaa < 1:2593001/69‑1 (MQ=255) gTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg > 1:2352766/1‑69 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:1902732/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:2172956/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:1699310/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:2235898/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:258502/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:2774974/69‑1 (MQ=255) aTGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:3116938/68‑1 (MQ=255) | CTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGA > minE/2627391‑2627522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |