| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,627,458 | C→T | 30.6% | intergenic (+198/‑73) | rpoH → / → livJ | RNA polymerase, sigma 32 (sigma H) factor/leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,627,458 | 0 | C | T | 30.6% | 126.5 / 62.4 | 130 | intergenic (+198/‑73) | rpoH/livJ | RNA polymerase, sigma 32 (sigma H) factor/leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (53/37); new base T (29/11); total (82/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
Only 100 of 166 total aligned reads displayed.
CTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGATT > minE/2627391‑2627524 | cTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGc > 1:1281061/1‑68 (MQ=255)cTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGc > 1:1828418/1‑68 (MQ=255) atCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaa > 1:2876972/1‑68 (MQ=255) atCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaa > 1:1317310/1‑68 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2919187/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2555497/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:876819/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2713451/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:86059/69‑1 (MQ=255) tCATAAAAATGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGCTACGTATAAAACGAAAt < 1:2287792/69‑1 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:1723230/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:3310123/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:3134148/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:1081325/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:2019809/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:2753797/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:2977360/1‑68 (MQ=255) tCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGaaa > 1:1107181/1‑68 (MQ=255) aaaaTGGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:2676513/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:600934/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:1423567/1‑69 (MQ=255) aaaaTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGt > 1:1920309/1‑69 (MQ=255) aTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGc > 1:2314334/1‑68 (MQ=255) aTCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGc > 1:2934754/1‑68 (MQ=255) aTGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaac < 1:841115/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:1991927/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:1124701/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:2862916/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:1253461/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:134756/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:3109676/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:2508800/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:2395650/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:1717575/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac > 1:538281/1‑69 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:1851147/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaac < 1:500255/69‑1 (MQ=255) ttttAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAaacaa < 1:424749/68‑1 (MQ=255) agagTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCacaa < 1:2214616/69‑1 (MQ=255) agagTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCacaa < 1:2450874/69‑1 (MQ=255) agagTATGCTGCTAAAGCACGGGTACTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCacaa < 1:792363/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:1172861/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:405143/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:538671/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:2481067/69‑1 (MQ=255) tATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacaca < 1:2966794/69‑1 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:3033120/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:3022089/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:417262/1‑68 (MQ=255) tgctgcTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAacacac > 1:3399875/1‑68 (MQ=255) ctgctAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGt < 1:2604261/68‑1 (MQ=255) ctgctAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGt > 1:2834637/1‑68 (MQ=255) ctgctAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGt < 1:1831317/68‑1 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGGACAACAACATCACAacacac > 1:3160496/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACTTCACAacacac > 1:2554691/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAataca > 1:878634/1‑56 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:2659112/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:309436/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:550690/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:370838/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:3173566/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:327869/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:3354798/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1343352/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1499013/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:2539198/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1687087/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:2428003/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:1777977/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacacac > 1:2225268/1‑57 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATCACAacaca > 1:2934237/1‑56 (MQ=255) cACGGGTAGCTACGTATAAAACGAAATAAAGTGCTGCACAACAACATAACAacacac > 1:3193433/1‑57 (MQ=255) ggTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAgaagaa < 1:1119225/69‑1 (MQ=255) ggTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAgaagaa < 1:2593001/69‑1 (MQ=255) gTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg > 1:2352766/1‑69 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:2774974/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:1699310/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:1902732/69‑1 (MQ=255) cATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGa < 1:2172956/69‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATca > 1:1523486/1‑35 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGta > 1:2637923/1‑46 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:3364752/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg > 1:943018/1‑63 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:2688581/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:803014/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:1584043/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:741357/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:594508/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg > 1:2462366/1‑63 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:3257480/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:326157/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:2054670/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:2094616/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:2340374/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATggg < 1:2305021/63‑1 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGAtt > 1:1669350/1‑67 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGAtt > 1:2364120/1‑67 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGAtt > 1:84005/1‑67 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGAtt > 1:846191/1‑67 (MQ=255) cATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGAtt > 1:2665606/1‑67 (MQ=255) | CTATTCCAATATCATAAAAATCGGGTATGTTTTAGCAGAGTATGCTGCTAAAGCACGGGTAGTCATGCATAAAACGAAATAAAGTGCTGAAAAACAACATCACAACACACGTAATAACCAGAAGAATGGGGATT > minE/2627391‑2627524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |