| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,627,716 | T→C | 27.3% | I62I (ATT→ATC) | livJ → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,627,716 | 0 | T | C | 27.3% | 89.7 / 33.8 | 84 | I62I (ATT→ATC) | livJ | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (43/18); new base C (11/12); total (54/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01 | |||||||||||
AGTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGC > minE/2627649‑2627778 | aGTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTa < 1:2478350/69‑1 (MQ=255)aGTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTa < 1:303625/69‑1 (MQ=255) gTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTaa > 1:2293922/1‑69 (MQ=255) gTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTaa > 1:709507/1‑69 (MQ=255) gTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTaa > 1:635529/1‑69 (MQ=255) gTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTaa > 1:58305/1‑69 (MQ=255) gTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTaa > 1:3395974/1‑69 (MQ=255) gTACGGTGACCAGGAGGTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTaa > 1:2490717/1‑69 (MQ=255) aCGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTaaa > 1:3393415/1‑68 (MQ=255) cGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAg > 1:2466989/1‑68 (MQ=255) cGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAg > 1:1500980/1‑68 (MQ=255) cGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAg > 1:3205523/1‑68 (MQ=255) cGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAg > 1:3058623/1‑68 (MQ=255) gTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGc < 1:3127653/68‑1 (MQ=255) aCCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGcaaca < 1:993730/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:3155579/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:674234/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:2855373/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:683802/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:938557/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:939094/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:2838061/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:2206665/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:2183156/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:1171060/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:1595870/69‑1 (MQ=255) aGGAGTTTACTGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAAc < 1:186553/69‑1 (MQ=255) gTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCa > 1:1440415/1‑69 (MQ=255) gTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCa > 1:1509812/1‑69 (MQ=255) gTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCa > 1:490858/1‑69 (MQ=255) gTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCa > 1:2844070/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:1955485/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:2147211/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:1079488/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:842715/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:772133/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:67877/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:3278630/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:2861789/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:2777476/1‑69 (MQ=255) tACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAAt > 1:2097566/1‑69 (MQ=255) tACCGGAGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCAtt > 1:6098/1‑49 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAGGGCAACAAACTGCAAATCg > 1:2125563/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:3390428/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:2069736/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:667680/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:522364/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:2538341/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:2759442/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:1700805/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:2869925/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGCAACAAACTGCAAATCg > 1:1859017/2‑68 (MQ=255) tggCGCAGAGCAGGCGGTTGCGGATATCAATGCTAAAGGTGGTATCAAAGGAAACAAACTGCAAATCg > 1:3372450/2‑68 (MQ=255) cGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGTCGGCATTAAAGGcaact < 1:846767/56‑2 (MQ=255) cAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGAc < 1:2545670/69‑1 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGTCGATGCCtgt > 1:362126/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCtgt > 1:1348027/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCtgt > 1:47390/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCtgt > 1:2080066/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCtgt > 1:1630321/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCtgt > 1:1046450/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCtgt > 1:311844/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCtgt > 1:3093772/1‑69 (MQ=255) gCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTCCAAATCGTAAAATATGACGATGCCtgt > 1:1407478/1‑69 (MQ=255) atCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGAccc > 1:2984028/1‑68 (MQ=255) aaCGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGa > 1:3137931/1‑67 (MQ=255) aaCGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGa > 1:1920010/1‑67 (MQ=255) cTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAgg > 1:2562837/1‑69 (MQ=255) cTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAgg > 1:2588866/1‑69 (MQ=255) cTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAgg > 1:2425867/1‑69 (MQ=255) cTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAgg > 1:155557/1‑69 (MQ=255) cTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAgg > 1:2929862/1‑69 (MQ=255) cTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAgg > 1:3048615/1‑69 (MQ=255) gcggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGa < 1:2687608/57‑1 (MQ=255) gcggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGa < 1:2041899/57‑1 (MQ=255) gcggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGa < 1:169989/57‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATTCCTGTGACCCGAAACAGGCGGTTGc < 1:1328322/69‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:674206/69‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:2683963/69‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:1449484/69‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:2287639/69‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:3203513/69‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:847537/69‑1 (MQ=255) cggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:1757572/69‑1 (MQ=255) ggcATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGc < 1:3271665/68‑1 (MQ=255) | AGTACGGTGACCAGGAGTTTACCGGCGCAGAGCAGGCGGTTGCGGATATCAACGCTAAAGGCGGCATTAAAGGCAACAAACTGCAAATCGTAAAATATGACGATGCCTGTGACCCGAAACAGGCGGTTGC > minE/2627649‑2627778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |