| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,628,280 | T→C | 73.3% | G250G (GGT→GGC) | livJ → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,628,280 | 0 | T | C | 73.3% | 72.2 / 23.4 | 67 | G250G (GGT→GGC) | livJ | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (7/11); new base C (38/11); total (45/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCCTGCGTCAGGCACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGA > minE/2628223‑2628347 | aaTCCTGCGTCAGGCACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGt < 1:2847762/69‑1 (MQ=255) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:2840951/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:203066/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:3325578/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:1836526/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:329331/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:473343/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:3034606/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:151484/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:1359807/69‑1 (MQ=25) cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:927996/69‑1 (MQ=25) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTaag > 1:3142369/1‑68 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1414155/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:2864417/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:2769484/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:59129/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1765653/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1816423/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1346686/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1221242/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:3396672/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:2092205/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCGCGCTGTCTAAc > 1:3104365/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGACTCGCTGTCTAAc > 1:1191590/1‑69 (MQ=21) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGgtgt > 1:540329/1‑46 (MQ=255) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:1904070/1‑69 (MQ=255) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:2147406/1‑69 (MQ=255) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:325848/1‑69 (MQ=255) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:1692091/1‑69 (MQ=255) cgGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGGGGCTAACGTCTCGCTGTCt < 1:87247/59‑1 (MQ=21) aaaCTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGc > 1:1830383/1‑67 (MQ=255) aaaCTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGc > 1:492646/1‑67 (MQ=255) aaCTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGa > 1:1591918/1‑69 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:3281494/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:3166888/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:1792731/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:2803986/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTATAACATTGCGGGCGAATCAGCGGAAGGgc < 1:2197056/67‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:2982148/69‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:966135/69‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:1299950/69‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:2346607/69‑1 (MQ=255) aGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:2515148/68‑1 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGca > 1:2327530/1‑45 (MQ=37) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2784736/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:922711/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:913585/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:893077/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1382379/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:827712/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1402698/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:570191/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1783928/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:447446/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1930439/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2146829/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2164269/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2411219/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2441131/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:3067129/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2637848/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:3028793/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2730266/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:280416/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGG‑‑‑GCTGGTGACCAAGCCga > 1:1550935/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTATAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1775779/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTATAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:3234008/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTCTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1177197/1‑68 (MQ=255) | AATCCTGCGTCAGGCACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGA > minE/2628223‑2628347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |