| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,628,292 | T→C | 63.8% | V254V (GTT→GTC) | livJ → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,628,292 | 0 | T | C | 63.8% | 47.9 / 43.4 | 77 | V254V (GTT→GTC) | livJ | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (16/12); new base C (38/11); total (54/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.36e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGCACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACG > minE/2628233‑2628356 | cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:473343/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:329331/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:203066/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:1836526/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:3325578/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:3034606/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:151484/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:1359807/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:2840951/69‑1 (MQ=25)cAGGCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCt < 1:927996/69‑1 (MQ=25) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTaag > 1:3142369/1‑68 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:59129/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:2864417/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1414155/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1346686/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1765653/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:3396672/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1816423/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:2092205/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:1221242/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCTCGCTGTCTAAc > 1:2769484/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGTCGCGCTGTCTAAc > 1:3104365/1‑69 (MQ=21) gCACGTGCGGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGTGGCTAACGACTCGCTGTCTAAc > 1:1191590/1‑69 (MQ=21) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:1904070/1‑69 (MQ=255) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:325848/1‑69 (MQ=255) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:2147406/1‑69 (MQ=255) cACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACa > 1:1692091/1‑69 (MQ=255) cgGCAGGGCTGAAAACCCAGTTTATGGGGCCGGAAGGCGGGGCTAACGTCTCGCTGTCt < 1:87247/59‑1 (MQ=21) aaaCTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGc > 1:1830383/1‑67 (MQ=255) aaaCTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGc > 1:492646/1‑67 (MQ=255) aaCTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGa > 1:1591918/1‑69 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:3281494/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:3166888/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:1792731/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGgc < 1:2803986/67‑1 (MQ=255) tttATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTATAACATTGCGGGCGAATCAGCGGAAGGgc < 1:2197056/67‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:2982148/69‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:966135/69‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:1299950/69‑1 (MQ=255) aaGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:2346607/69‑1 (MQ=255) aGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGc < 1:2515148/68‑1 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGca > 1:2327530/1‑45 (MQ=37) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2730266/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:447446/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1402698/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:570191/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1783928/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1382379/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1930439/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:827712/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2146829/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2164269/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:893077/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:3067129/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:913585/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:3028793/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:922711/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2411219/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2441131/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:280416/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2784736/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:2637848/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTCTAACATTGCGGGTGAATCAGCAGAAGG‑‑‑GCTGGTGACCAAGCCga > 1:1550935/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTATAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:3234008/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTGTATAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1775779/1‑68 (MQ=255) aGGCGTGGCTAACGTCTCGCTCTCTAACATTGCGGGTGAATCAGCAGAAGGGCTGCTGGTGACCAAGc > 1:1177197/1‑68 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:3336569/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:3296265/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:747179/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:2136572/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:2290426/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:305324/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:2411146/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:2794430/1‑69 (MQ=255) gCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAAc > 1:2521148/1‑69 (MQ=255) gTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACg < 1:379559/67‑1 (MQ=255) gTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACg < 1:1322212/67‑1 (MQ=255) | CAGGCACGCGCGGCAGGGCTGAAAACTCAGTTTATGGGGCCGGAAGGTGTGGCTAACGTTTCGCTGTCTAACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACG > minE/2628233‑2628356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |