| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 224,081 | G→T | 44.2% | A338A (GCC→GCA) | pepD ← | aminoacyl‑histidine dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 224,081 | 0 | G | T | 44.2% | 38.7 / 71.4 | 79 | A338A (GCC→GCA) | pepD | aminoacyl‑histidine dipeptidase |
| Reads supporting (aligned to +/- strand): ref base G (17/27); new base T (17/18); total (34/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGA > minE/224013‑224147 | tGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTg < 1:2887482/69‑1 (MQ=255)tGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTg < 1:344637/69‑1 (MQ=255)tGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTg < 1:1739438/69‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:1585655/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:2123258/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:230761/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:2309777/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:2343708/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:1971727/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:2839255/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:2902742/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:294265/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:3061635/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:368695/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:132630/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:853075/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:883151/66‑1 (MQ=255) ttATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:939391/66‑1 (MQ=255) ttttGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:3202044/63‑1 (MQ=255) tATTGTCGGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCg < 1:2220054/65‑1 (MQ=255) aTTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGtt > 1:1638182/1‑69 (MQ=255) aTTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGtt > 1:1491863/1‑69 (MQ=255) aTTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGtt > 1:991492/1‑69 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:2610103/69‑1 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:3197014/69‑1 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:804585/69‑1 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:2147632/69‑1 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:1502096/69‑1 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:1522504/69‑1 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:1433762/69‑1 (MQ=255) tcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca < 1:1885387/69‑1 (MQ=255) caTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac > 1:3422128/1‑69 (MQ=255) caTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac > 1:972795/1‑69 (MQ=255) caTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac > 1:1988690/1‑69 (MQ=255) caTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac > 1:3115915/1‑69 (MQ=255) caTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac > 1:2842390/1‑69 (MQ=255) caTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac > 1:596874/1‑69 (MQ=255) caTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac > 1:1291840/1‑69 (MQ=255) cacaCCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCGACGTCg < 1:613716/46‑1 (MQ=37) cacCGACGTTCAGGGAGGTTTCGCCCACACCTTTTGCCACGTCGGAGTTGAGGATCACACCGTTCgggg > 1:2797708/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGTTCACACCGTTCgggg > 1:2388271/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:409882/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:2036362/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:1493983/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:2011496/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:2336890/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:198830/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:2981578/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:207497/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:1439515/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:1902245/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:2509527/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:701470/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:76017/1‑69 (MQ=255) cacCGACGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCggg > 1:2968440/1‑68 (MQ=255) cacCGAAGTTCAGGGAGGTTTCGACCACACCTTTTGCCACGTCGGAGTTGCGGATCACACCGTTCgggg > 1:439860/1‑69 (MQ=255) gAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCgg > 1:2384751/1‑53 (MQ=255) gAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcagcag > 1:840988/1‑68 (MQ=255) gAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcagcag > 1:2969346/1‑68 (MQ=255) gAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcagcag > 1:2556935/1‑68 (MQ=255) ttCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGaa > 1:2592703/1‑68 (MQ=255) tCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATa < 1:1168803/69‑1 (MQ=255) tCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATa < 1:2962544/69‑1 (MQ=255) tCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATa < 1:3255515/69‑1 (MQ=255) tCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATa < 1:2832539/69‑1 (MQ=255) tCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATa < 1:2700986/69‑1 (MQ=255) tCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATa < 1:2691994/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATc > 1:1269815/1‑65 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATc > 1:111847/1‑65 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:1165435/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:2265793/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:1168352/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:2471120/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:2603108/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:723280/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:695261/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:2725793/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:2815058/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:1866948/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:388463/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:2921829/69‑1 (MQ=255) ttGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCga < 1:1635060/69‑1 (MQ=255) | TGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGA > minE/224013‑224147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |